| 卵巢癌高频转移细胞模型中nm23-H1基因表达的相关性研究 |
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| 引用本文： | 高庆蕾,马丁,孟力,王世宣,王常玉,芦运萍,张阿丽,李静. 卵巢癌高频转移细胞模型中nm23-H1基因表达的相关性研究[J]. 中国肿瘤临床(英文版), 2004, 0(2) |
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| 作者姓名： | 高庆蕾 马丁 孟力 王世宣 王常玉 芦运萍 张阿丽 李静 |
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| 作者单位： | 武汉华中科技大学同济医学院附属同济医院妇产科 430030 |
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| 基金项目： | This work was supported by the grants from 973 National Great Foundation Research Program of China(No.2002CB513100)，the National Prominent Youth Foundation of China(No.30025017). |
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| 摘 要： | 目的筛选高频转移卵巢恶性肿瘤细胞,研究不同转移潜能的细胞和 nm23的相关性。方法通过反复动物接种和体外培养,观察动物肺转移状况,筛选高频转移细胞株,比较原发肿瘤和转移肿瘤的特征,并应用 North-ern-blot 方法测定各类肿瘤细胞 nm23 mRNA 表达水平。结果 8株卵巢恶性肿瘤细胞中4株有较高转移潜能。多次培养接种可筛选出高频转移细胞亚群。测定各类细胞 nm23 mRNA 表达水平与肿瘤转移特性呈负相关。结论由基因分子水平决定的肿瘤转移趋势在不同肿瘤种类及细胞亚群中有明显差异;卵巢癌中 nm23 mRNA 和蛋白的表达与其转移能力的降低有密切关系,可作为判定卵巢癌预后的敏感指标。
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| 关 键 词： | 卵巢恶性肿瘤 肿瘤转移 nm23 |
Related Study on the Nm23-H1 Gene Expression in the Model of Ovarian Carcinoma Cell Lines with High Frequent Metastasis |
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| GAO Qinglei MA Ding WANG Shixuan WANG Changyu LU Yunping ZHANG Ali LI Jing. Related Study on the Nm23-H1 Gene Expression in the Model of Ovarian Carcinoma Cell Lines with High Frequent Metastasis[J]. Chinese Journal of Clinical Oncology, 2004, 0(2) |
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| Authors: | GAO Qinglei MA Ding WANG Shixuan WANG Changyu LU Yunping ZHANG Ali LI Jing |
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| Affiliation: | GAO Qinglei MA Ding WANG Shixuan WANG Changyu LU Yunping ZHANG Ali LI Jing Department of Gynecology and Obstetrics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,ChinaMENG Li Southwestern Medical Center,USA |
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| Abstract: | Objective:To select the ovarian carcinoma cell lines with high frequent metastasis and study the association between nm23-H1 gene expression and metastasis of ovarian carcinoma.Methods:Each ovarian cancer cell line was transplanted subcutaneously into the flank of nude mice,and the metastatic behavior was evaluated by counting lung tumor foci at different time points.The metastatic tumors were cultured in vitro,then substrain was established and transplanted subcutaneously three times.The RNA level of nm23 in 8 human ovarian cancer cell lines were examined by northern-blot.Results:Of the 8 human ovarian cancer cell lines,4 had high frequent metastatic potentiality.The subpopulation of cells with high frequent metastasis could be screened by injection several times.The expression of nm23 RNA in human ovarian cancer cells was inversely related to metastatic behavior in the experimental animals (r=0.96,P=0.0001).Conclusion:The difference of the tendency of metastasis which was determined by genetic and molecular levels was significant among different type of cell lines and subtypes.The expression of nm23 mRNA in human ovarian carcinomas was correlated closely with the reduced metastatic behavior in experimental animals and may serve as a sensitive prognostic indicator for ovarian cancer. |
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| Keywords: | ovarian carcinoma metastasis nm23 |
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