氨基胍与环孢素A联用对大鼠心脏移植后急性排斥反应的影响

目的：探讨氨基胍与环孢素A联用对同种大鼠心脏移植后急性排斥反应的影响。方法：受体SD大鼠心脏移植后分为4组：（1）对照组：术后不作任何处理；（2）低剂量环孢素A（CsA)组；术后0－7d肌肉注射CsA2mg.kg^-1.d^-1；（3）氨基胍（AG）组：术后0－7d皮下注射AG600mg.kg^-1.d^-1;（4）低剂量CsA加AG组；术后0－7d肌肉注射CsA2mg.kg^-1.d^-1及皮下注射AG600mg.kg^-1.d^-1。术后4d测定急性排斥反应时移植心的诱生型一氧化氮合酶（iNOS)的表达及血清一氧化氮（NO）含量，并观察移植心存活时间。结果与低剂量环孢素A组相比较，低剂量环孢素A与氨基胍联用组不仅显著地抑制移植心iNOS表达与NO产生（P＜0．05）；而且显著地减轻急性排斥反应（P＜0．01），延长了移植心存活时间（P＜0．05）。结论低剂量环孢素A与氨基胍联用，协同抑制急性排斥反应时移植心iNOS活性及NO产生；显著地延长移植物存活时间。

Effect of combined use of aminoguanidine and cyclosporine A on acute rejection following cardiac allograft in rats

Objective To study the effect of the combined use of aminoguanidine (AG) and cyclosporine A (CsA) on acute rejection following cardiac allograft. Methods Except the control group, all other three groups were respectively treated by AG 600?mg/kg every day subcutaneously 0 to 7 days after transplantation, low dose CsA 2?mg/kg every day i.m 0 to 7 days after transplantation, and low dose CsA plus AG. Inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production during acute allograft rejection were detected at 4th day after operation, and the graft survival was observed.Results Compared with the control group, iNOS expression and NO production were significantly inhibited in the AG treated group ( P < 0.05 ),the progression of acute rejection was slightly attenuated and allografted heart survival prolonged. The inhibitory effects of iNOS activity and NO production in the recipients receiving the combined treatment of low dose CsA plus AG were higher than those receiving low dose CsA alone. Compared with the low-dose CsA group, low dose CsA plus AG could significantly reduce the histologic grade of acute rejection ( P < 0.01 ) and prolong the graft survival ( P < 0.05 ). Conclusion The combined use of low dose CsA and AG could synergistically suppress iNOS activity and NO production, and significantly prolong allografted heart survival.