| Aresearch on DLA－DRB1 Genotyping by PCR－RFLP ）I． ToSelect a Appropriate Oligonucleotide Primer Pair |
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| 引用本文： | 贺水文. Aresearch on DLA－DRB1 Genotyping by PCR－RFLP ）I． ToSelect a Appropriate Oligonucleotide Primer Pair[J]. 华中科技大学学报(医学英德文版), 1994, 14(1): 24-28. DOI: 10.1007/BF02888053 |
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| 作者姓名： | 贺水文 |
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| 作者单位： | Department of Infectious Diseases，Xiehe Hospital，Tongji Medical University，Wukan Department of Immunology，University Hospital of Essen，Essen，Federal Republic of Germany |
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| 摘 要： | （贺水文）ＡｒｅｓｅａｒｃｈｏｎＤＬＡ－ＤＲＢ１ＧｅｎｏｔｙｐｉｎｇｂｙＰＣＲ－ＲＦＬＰＩ．ＴｏＳｅｌｅｃｔａＡｐｐｒｏｐｒｉａｔｅＯｌｉｇｏｎｕｃｌｅｏｔｉｄｅＰｒｉｍｅｒＰａｉｒ￥ＨＥＹｏｎｇ－ｗｅｎ，ＦｅｒｅｎｃｉｋＳ，Ｇｒｏｓｓｅ－Ｗｉｌｄｅ...
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| 关 键 词： | PCR-RFLP primer DLA class Ⅱ antigens |
| 收稿时间： | 1993-02-08 |
A research on DLA-DRB1 genotyping by PCR-RFLP I. To select a appropriate oligonucleotide primer pair |
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| He Yong-wen,S. Ferencik,H. Grosse-Wilde. A research on DLA-DRB1 genotyping by PCR-RFLP I. To select a appropriate oligonucleotide primer pair[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 1994, 14(1): 24-28. DOI: 10.1007/BF02888053 |
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| Authors: | He Yong-wen S. Ferencik H. Grosse-Wilde |
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| Affiliation: | (1) Department of Infectious Diseases, Xiehe Hospital, Tongji Medical University, Wuhan;(2) Department of Immunology, University Hospital of Essen, Essen, Germany |
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| Abstract: | Summary In order to study the DLA (Dog Leucocyte Antigen) classI region we utilized the polymerase chain reaction based restriction fragment length polymorphism (PCR-RFLP) method, which has been reported previously as an efficient and simple technique for accurate definition of the HLA classI alleles[1]. To search for a appropriate primer pair a serie of amplifications with 4 different primer pairs DLA-DR-SP/Stop, DLA-DR-SP/P3, HLA-DRB-GH46/50 and HLA-DRB-AMP-A/B were provided. Only one satisfactory amplification was obtained with the primer pair HLA-DRB-AMP-A/B. The analogous sequences of the primer pair are found in the sequence of HLA-DRB-cDNA. The amplification region of the primer pair includes also the three hypervariable regions (HVR) in the sequence of DLA-DRB cDNA. Southern blot hybridization analysis confirmed the specificity of the primer pair HLA-DRB-AMP-A/B. The results of HaeI and Hinfl digestion show high polymorphism in DLA-D region and allele specific polymorphic patterns. Therefore, it is suggested that the primer pair HLA-DRB-AMP-A/B is at present the only available and usefull primer pair in PCR-RFLP study of DLA-DRB1 gene. |
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| Keywords: | PCR-RFLP primer DLA classI antigens |
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