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| 绿原酸完全抗原的制备及鉴定 | |
| 引用本文： | 徐国良,张增珠,张卓辉,张启云,李敏,刘旭海. 绿原酸完全抗原的制备及鉴定[J]. 卫生研究, 2008, 37(6) |
| 作者姓名： | 徐国良 张增珠 张卓辉 张启云 李敏 刘旭海 |
| 作者单位： | 1. 江西中医学院现代中药制剂教育部重点实验室,南昌,330004 2. 中国人民解放军第九四医院 3. 江中药业股份有限公司 |
| 基金项目： | 江西省卫生厅中医药科研基金项目 |
| 摘 要： | 目的制备绿原酸完全抗原,对完全抗原进行鉴定。方法通过碳二亚胺(EDC)法把绿原酸与载体蛋白牛血清白蛋白和卵蛋白进行偶联制备,采用紫外扫描法对合成抗原进行了鉴定;用绿原酸完全抗原免疫豚鼠,设生理盐水对照组,绿原酸标准溶液对照组。间接酶联免疫法测定免疫豚鼠抗血清的效价。结果完全抗原偶联成功,测定偶联比20,符合动物免疫的要求。ELISA法检测豚鼠抗血清效价,完全抗原组豚鼠抗血清稀释1∶128为阳性,大于空白复孔A值2倍。结论成功制备绿原酸完全抗原。 |
| 关 键 词： | 绿原酸 完全抗原 制备和鉴定 中药材 |
Preparation and characterization of chlorogenic acid complete antigen |
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| XU Guoliang,ZHANG Zengzhu ZHANG Zhuohui,ZHANG Qiyun,et al.. Preparation and characterization of chlorogenic acid complete antigen[J]. Journal of hygiene research, 2008, 37(6) | |
| Authors: | XU Guoliang ZHANG Zengzhu ZHANG Zhuohui ZHANG Qiyun et al. |
| Affiliation: | XU Guoliang,ZHANG Zengzhu ZHANG Zhuohui,ZHANG Qiyun,et al.Jiangxi University of Traditional Chinese Medicine Modern Chinese Native Medicine Preparation Ministry of Education key laboratory,Nanchang 330004,China |
| Abstract: | Objective Characterizate the complete antigen of the chlorogenic acid.Methods The chlorogenic acid was coupled to BSA as an immunogen and to OVA as a coating antigen using the coupling agent EDC.HCL The immunogen and coating antigen were characterizated by the ultraviolet scanner.the complete antigen was uesd to immnune the cony pig,Detect the antiserum by ELISA.Results The coupling ratio of the complete antigens was 20.the quinza pig antiserums in 1∶128 in OD values were more higher than 2 times of the negative comparison.Conclusion The complete antigen was preparated successfully |
| Keywords: | chlorogenic acid complete antigen characterizate and prerepation |
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