| 人表皮细胞的体外改良培养 |
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| 引用本文： | 冯若,金辉,杨继要.人表皮细胞的体外改良培养[J].河南医学研究,2005,14(2):129-130. |
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| 作者姓名： | 冯若 金辉 杨继要 |
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| 作者单位： | 郑州大学基础医学院组织学与胚胎学教研室,河南,郑州,450052;郑州大学基础医学院组织学与胚胎学教研室,河南,郑州,450052;郑州大学基础医学院组织学与胚胎学教研室,河南,郑州,450052 |
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| 摘 要： | 目的:探讨人工纯化法去除成纤维细胞对人表皮细胞生长的影响。方法:原代培养人表皮细胞第5天时,采用人工纯化法即用0.25%胰蛋白酶+0.04%EDTA(1∶1)消化细胞2～10min,除去成纤维细胞后,观察表皮细胞生长情况并用台盼蓝染色检测其存活率。结果:细胞存活率为87%,存活率较高。生长状态较传统方法培养的表皮细胞生长状态好。结论:人工纯化法除去成纤维细胞为原代培养表皮细胞的方法改良,有利于表皮细胞更好的生长。
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| 关 键 词： | 细胞培养 表皮细胞 改良方法 |
| 文章编号： | 1004-437X(2005)02-0129-02 |
| 修稿时间： | 2005年3月25日 |
An improved method to culture epidermal cells |
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| FENG Ruo,JIN Hui,YANG Ji-yao.An improved method to culture epidermal cells[J].Henan Medical Research,2005,14(2):129-130. |
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| Authors: | FENG Ruo JIN Hui YANG Ji-yao |
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| Abstract: | Objective: To investigate an improved method to culture epidermal cells.Methods: To remove fibroblasts,epidermal cells were manually purified by 0.25% trypsin plus 0.04% EDTA(1∶1).The growing state of epidermal cells was observed and the survival rate of epidermal cells was detected by tryphan blue staining.Results: The survival rate of epidermal cells was 87%.The growing state of epidermal cells cultured after manually purification was better than that of epidermal cells cultured by traditional methods.Conclusion: Manually purification to remove fibroblasts is an improved method to culture epidermal cells primarily. |
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| Keywords: | cell culture epidermal cell improved method |
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