实时荧光PCR法检测正常人脑膜炎奈瑟菌

目的建立一种简易快速的实时荧光PCR方法（Q—PCR）并用于检测正常人群中脑膜炎奈瑟菌。方法采集成都市正常人群的咽拭子1008份，分别采用培养分离法和Q—PCR法检测Nm。绪果Q—PCR法方法的灵敏度、特异性分别为100％和100％，阴性预测值（NPV）为100．00％，阳性预测值13．09％，检出限为250du／ml体系，Q—PCR方法检出时间为3h，培养法阳性结果需时4d。Q—PCR技术检测1008份正常人群的咽拭子中NmDNA的阳性率为8．33％，培养法为1．09％。结论Q—PCR技术的灵敏度高于培养法，Q—PCR法具有灵敏、特异、经济、快速的特点，可用于疾病的快速诊断，特别适合正常人群的带菌率调查。

Detection of Neisseria meningitides in Healthy Population by Real Time Quantitative PCR

Objective To develop a rapid test of real time quantitative PCR to detect Neisseria meningitides in healthy population. Methods A total of 1 008 throat swabs were taken in healthy population in Chengdu, Neisseria meningitides was detected by SYBR Green real-time PCR and culture/isolation assay. Results Both sensitivity and specificity of real time quantitative PCR were 100%. The negative predictive value was 100%, and the positive predictive value was 13.09%. The cut - off value of the assay was 250cfu/ml. The positive rate of Neisseria meningitides DNA by real time quantitative PCR was 8. 33% and the positive rate of Neisseria meningitides by culture/isolation was 1.09%. Conclution The real-time quantitative PCR is a rapid, sensitive and specific assay for the detection of Neisseria meningitides in healthy population, which could be a valuable method for the further understanding of the epidemiology of meningococcal infection.