| 熊果酸诱导肺腺癌SPC-A-1细胞凋亡及其机制 |
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| 引用本文： | 王静,王尉平,顾振纶,梁中琴,周文轩,郭次仪.熊果酸诱导肺腺癌SPC-A-1细胞凋亡及其机制[J].苏州大学学报(自然科学版),2008,28(2):207-210. |
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| 作者姓名： | 王静 王尉平 顾振纶 梁中琴 周文轩 郭次仪 |
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| 作者单位： | [1]苏州大学医学部生物化学与分子生物学教研室,江苏苏州215123 [2]苏州中药研究所,江苏苏州215007 |
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| 摘 要： | 目的 研究熊果酸(UA)对人肺腺癌SPC-A-1细胞凋亡的影响及其作用机制.方法 应用MTT法检测UA对SPC-A-1细胞增殖的作用.透射电镜观察细胞形态学变化.流式细胞仪(FCM)分析细胞周期和细胞凋亡率.RT-PCR探讨UA对SPC-A-1细胞GDF15和p21基因表达的影响.结果 UA抑制SPC-A-1细胞增殖,并呈浓度和时间依赖性.透射电镜观察UA作用的SPC-A-1细胞,出现胞质大量空泡变性、细胞核凝集等细胞凋亡形态学改变.流式细胞术检测到凋亡峰.30、40、50μmol/L的UA作用SPC-A-1细胞48h后,其凋亡率分别为1.83%、18.6%、22.6%,细胞周期阻滞于S期,且呈浓度依赖性.RT-PCR证实UA能使SPC-A-1细胞GDF15、p21基因表达上调.结论 UA对SPC-A-1细胞具有抑制增殖和诱导凋亡作用,其机制可能与上调GDF15和p21基因表达相关.
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| 关 键 词： | 熊果酸 SPC-A-1细胞 周期阻滞 凋亡 GDF15 p21 酸诱导 肺腺癌 细胞凋亡率 机制 Cells Lung Adenocarcinoma Human Ursolic Acid Induced Apoptosis 相关 凋亡作用 抑制增殖 表达上调 基因表达 浓度依赖性 细胞周期阻滞 凋亡峰 流式细胞术检测 |
| 文章编号： | 1673-0399(2008)02-0207-04 |
| 修稿时间： | 2007年4月28日 |
Mechanisms of Apoptosis Induced by Ursolic Acid in Human Adenocarcinoma of Lung SPC-A-1 Cells |
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| WANG Jing,WANG Wei-ping,GU Zhen-lun,LIANG Zhong-qin,ZHOU Wen-xuan ,GUO Ci-yi.Mechanisms of Apoptosis Induced by Ursolic Acid in Human Adenocarcinoma of Lung SPC-A-1 Cells[J].Suzhou University Journal of Medical Science,2008,28(2):207-210. |
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| Authors: | WANG Jing WANG Wei-ping GU Zhen-lun LIANG Zhong-qin ZHOU Wen-xuan GUO Ci-yi |
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| Institution: | WANG Jing1,2,WANG Wei-ping1,GU Zhen-lun2,LIANG Zhong-qin2,ZHOU Wen-xuan 2,GUO Ci-yi2 (1.Dept of Biochemistry , Molecular Biology,Medical College,Suzhou University,Jiangsu Suzhou 215123,China,2.Suzhou Institute of Chinese Materia Medica,Jiangsu Suzhou 215007,China) |
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| Abstract: | Objective To study the apoptosis effects of ursolic acid(UA) on human adenocarcinoma of lung SPC-A-1 cells and the involed mechanisms. Methods Cell proliferation of UA on SPC-A-1 cell was evaluated by MTT assay. The morphological changes were observed by electron microscope. Cell cycle and apoptosis rate were analysed by flow cytometry(FCM). The gene expression of GDF15, p21 involved in the effects of UA on SPC-A-1 cells was studied by RT-PCR. Results MTT assay showed that UA had a anti-proliferation effect... |
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| Keywords: | ursolic acid SPC-A-1 cycle arrest apoptosis GDF15 p21 |
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