| NGF诱导PC12细胞分化的神经细胞行为观察 |
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| 引用本文： | 丁明杰,史学义,张娓,丁一. NGF诱导PC12细胞分化的神经细胞行为观察[J]. 郑州大学学报(医学版), 2001, 36(3): 281-283 |
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| 作者姓名： | 丁明杰 史学义 张娓 丁一 |
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| 作者单位： | 河南医科大学组织学与胚胎学教研室 |
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| 基金项目： | 河南省教委自然科学基础研究项目!19993 10 0 0 8 |
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| 摘 要： | 目的 :观察PC12细胞分化的神经细胞的分裂、突起延伸、迁徙和分化等细胞行为。方法 :采用Giemsa、甲绿 派郎宁染色和增殖细胞核抗原 (PCNA)免疫组织化学染色方法观察培养的PC12细胞。结果 :培养分化的神经细胞呈现横裂、纵裂等不同方式的无丝分裂像 ;分裂中的神经细胞可呈现形态及化学不对称性 ;分化的神经细胞有明显的细胞迁徙运动和突起延伸。结论 :NGF诱导PC12细胞分化的神经细胞能以无丝分裂方式增殖 ;分化神经元可以通过细胞迁徙、突起延伸及共同胞质桥等方式相连接。
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| 关 键 词： | PC12细胞 神经细胞 无丝分裂 细胞分化 |
| 修稿时间： | 2000-09-06 |
Observation on differentiated neuron behaviour induced by NGF in cultured PC12 cells |
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| Ding Mingjie,SHI Xueyi,ZHANG Wei,DING Yi. Observation on differentiated neuron behaviour induced by NGF in cultured PC12 cells[J]. Journal of Zhengzhou University: Med Sci, 2001, 36(3): 281-283 |
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| Authors: | Ding Mingjie SHI Xueyi ZHANG Wei DING Yi |
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| Abstract: | Aim:To study the cell division, neurite extension, migration of neurons differentiated by NGF in subcultured PC12 cells.Methods: The Giemsa staining, methyl green pyronin staining and proliferating cell nuclear antigen immunohistochemical assay were used to study the subcultured PC12 cells.Results: The differentiated neuron appeared different patterns of amitotic figures such as transverse sever, longitudinal split, etc The proliferating neurons divided in an asymmetric way morphologically and chemically. The differentiated neuron appeared an obvious movement of cell migration and neurite extension.Conclusion: The neurons differentiated by NGF in cultured PC12 cells can divide in amitotic way.The differentiated neuron can form junction by cell migration, neurite extension or cytoplasmic bridge. |
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| Keywords: | PC12 cell neuron amitosis differentiation |
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