| 对脂多糖结合蛋白致炎的多肽序列分析 |
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| 引用本文： | 徐德斌,钱桂生,侯一峰,赵哓利,陈维中,李淑萍.对脂多糖结合蛋白致炎的多肽序列分析[J].重庆医学,2004,33(5):718-720. |
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| 作者姓名： | 徐德斌 钱桂生 侯一峰 赵哓利 陈维中 李淑萍 |
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| 作者单位： | 第三军医大学新桥医院全军呼吸内科研究所,重庆,400037;第三军医大学新桥医院全军呼吸内科研究所,重庆,400037;第三军医大学新桥医院全军呼吸内科研究所,重庆,400037;第三军医大学新桥医院全军呼吸内科研究所,重庆,400037;第三军医大学新桥医院全军呼吸内科研究所,重庆,400037;第三军医大学新桥医院全军呼吸内科研究所,重庆,400037 |
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| 摘 要： | 目的应用噬菌体随机12肽库探讨脂多糖结合蛋白(LBP)致炎作用的多肽序列.方法以LPS为目标分子,对噬菌体12肽库进行亲合筛选,4轮筛选后,检测随机挑取的噬菌体克隆的结合活性和抑制活性,将得到的16个可与LBP竞争性结合LPS的噬菌体克隆进行生物学功能检测,对获得的9个阳性克隆进行测序,并与LBP序列比较.结果9个阳性克隆展示多肽的核心序列为WKXRKXFXKXXG,与LBP的91～102位氨基酸序列有较高同源性.结论LBP的91～102位氨基酸可能为其致炎作用部位.
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| 关 键 词： | 噬菌体随机肽库 脂多糖结合蛋白 多肽 序列 |
| 文章编号： | 1671-8348(2004)05-0718-03 |
Analysis of inflammatory peptide sequence of lipopolysaccharide-binding protein |
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| XU De bin,QIAN Gui sheng,HOU Yi feng,et al..Analysis of inflammatory peptide sequence of lipopolysaccharide-binding protein[J].Chongqing Medical Journal,2004,33(5):718-720. |
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| Authors: | XU De bin QIAN Gui sheng HOU Yi feng |
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| Abstract: | Objective To determine the inflammatory peptide sequence of lipopolysaccharide binding protein(LBP).Methods Using LPS as target,4 rounds of bio panning to a phage random 12 mer peptide library were carried out.One hundred clones were selected and used in binding test,competitive inhibition test and function test.Positive clones were sequenced and compared with LBP.Results Sixteen phage clones could inhibit the binding of LBP to LPS,and 9 of which could inhibit the function of LBP which enhanced the LPS dependent activation of PBMC.The core sequence of 9 positive clones was WKXRKXFXKXXG.By homology analysis,one higher homologous sequence was found in LBP 91 102 aa.Conclusion The 91 102 aa of LBP could be inflammatory peptide sequence. |
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| Keywords: | phage random peptide library lipopolysaccharide binding protein peptide sequence |
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