功能性P2X1样受体在离体兔动脉的分布

目的:研究功能性P2X_1-like受体在兔6种动脉平滑肌的分布.方法:观察兔离体肾动脉(Re)、股动脉(Fe)、隐动脉(Sa)、肠系膜动脉(Me)、脾动脉(Sp)和耳动脉(Ea)环的收缩反应.结果:NA的最大收缩反应(E_(max·NA))值为Re>Fe>Sa>Me=SP>Ea;经氯化钾最大收缩反应(E_(max·KCl))标准化后,NA的标准化最大收缩反应(E_(max·NA)/E_(max·KCl))值,在 6种血管基本一致.α,β-Methylene ATP最大收缩反应(E_(max·α,β-meATP))值为 Re>Sa=Fe>Ea=Sp=Me;经E_(max·KCL)标准化后,α,β-methylene ATP的标准化最大收缩反应(E_(max·α,β-meATP)/E_(max·KCl))数值各血管仍不同,Fe

Distribution of functional P2X1-like receptor in isolated rabbit arteries

AIM: To clarify the distribution of functional P2X1-like receptors in rabbit arteries. METHODS: Isometric contractile responses to noradrenaline (NA) and alpha, beta-methylene ATP (alpha, beta-meATP) were observed in the arteries isolated from rabbit renal (Re), femoral (Fe), saphenous (Sa), mesenteric (Me), splenic (Sp), and ear (Ea). RESULTS: The maximal responses to NA (E(max.NA)) varied among the arteries in an order of Re>Fe>Sa>Me>Sp>Ea. After standardization by the maximal response to KCl (E(max.KCl)), however, the values of E(max.NA)/E(max.KCl) in the six kinds of arteries were almost the same. EC50 values of NA in arteries were different, and the EC50 value of NA in Me artery was 54 times that of Fe artery (P<0.01). The maximal response to alpha, beta-meATP (E(max.alpha, beta-meATP) ) varied among the arteries in an order of Re>Sa=Fe>Ea>Sp=Me, and the values of Emax.alpha, beta-meATP /E(max.KCl) were still different (Fe