不同海拔COPD患者血清基质金属蛋白酶-9和基质金属蛋白酶组织抑制剂-1的表达

目的通过ELISA法研究COPD患者血清基质金属蛋白酶-9（MMP-9）及其基质金属蛋白酶组织抑制剂-1（TIMP-1）在不同海拔中的表达。方法选取西宁COPD患者31例作为中度海拔组，同期玉树COPD患者24例作为高海拔组。各组均空腹采静脉血标本3mL静置1h后常温下离心（3000G）10min取血清。用双抗体夹心酶联免疫吸附试验（ELISA）测定MMP-9、TIMP—1。比较不同海拔地区COPD患者血清MMP-9、TIMP-1的差异。结果高海拔地区COPD组血清MMP-9、TIMP-1水平为64．198±8．800、10．715±0．630pg／mL；中度海拔地区COPD组血清MMP-9、TIMP-1水平为50．241±5．552、12．025±0．452pg／mL。不同海拔COPD患者血清MMP-9值相比存在显著性差异（P〈0．05）。不同海拔地区COPD患者血清TIMP-1值相比存在显著性差异（P〈0．05）。结论不同海拔COPD患者血清MMP-9、TIMP-1值存在差异。

A STUDY ON MMP- 9 AND TIMP- 1 IN DIFFERENT ALTITUDE IN PATIENTS WITH COPD

Objective serum matrix metalloproteinases - 9 （ MMP - 9 ） and tissue inhibitor of metalloproteinase （TIMP- 1 ）in different altitude in patients with COPD was investigated by ELISA. Methods 31 cases of patients with COPD in Xining as a moderate elevation group ,24 patients with COPD in Yushu as a high altitude group. Fast- ing venous blood 3 ml was taken respectively and stand for lh at room temperature under 3000G centrifugal 10min, using a double antibody sandwich enzyme immunosorbent assay （ELISA）to determine their level of MMP -9 and TIMP - 1 and the serum MMP -9 and TIMP - 1 in different altitudes in patients with COPD were compared. Result In the high altitudes,the expressions of MMP -9 and TIMP - 1 in the patients with COPD were 64. 198 ±8. 800 pg/mL and 10. 715± 0. 630 pg/mL, respectively. In the moderate altitudes, the expressions of MMP - 9 and TIMP - 1 in patients with COPD were 50. 241± 5. 552 pg/mL and 12. 025± 0.452 pg/mL, respectively. Both the level of MMP -9 and TIMP - 1 in patients with COPD in different altitude showed significant difference （ P 〈 0.05 ）. Conclusion The level of serum MMP - 9 and TIMP - 1 in patients with COPD in different altitude is difference.