Lysosomal and energy enzyme activities in hypertrophied rat soleus muscle after denervation |
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Authors: | A. Bass J. Teisinger P. Hnik E. V. Macková R. Vejsada I. Eržen |
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Affiliation: | (1) Institute of Physiology, Czechoslovak Academy of Sciences, Videská 1083, CS-142 20 Prague 4;(2) Institute of Hygiene and Epidemiology, Prague 10;(3) Research Institute of Physical Training and Sports, Charles University, Prague 1, Czechoslovakia;(4) Department of Anatomy, Medical Faculty, Ljubljana, Yugoslavia |
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Abstract: | Muscle hypertrophy was induced in the soleus muscle of young rats by tenotomy of the gastrocnemius and plantaris muscles. Three and 7 days afterwards the sciatic nerve was sectioned. The loss of weight of muscles subjected to this combined procedure three days after denervation was 30–40%. Lysosomal enzyme activities (acid phosphatase, -glucosidase, -galactosidase and N-acetyl--d-glucosaminidase) and energy enzyme activities (lactate dehydrogenase, LDH, triose-3-phosphate dehydrogenase, TPDH,d-hexokinase, HK and citrate synthase, CS) were determined 3 days after denervation, 3, 7 and 10 days after hypertrophy had been induced and 3 days after denervation of hypertrophying muscles on day 3 and 7. Normal non-operated rats of corresponding body wieght served as controls and their enzyme activities were estimated on the same day. In the course of muscle hypertrophy, the 4 lysosomal enzyme activities increased progressively. Although 3 days' denervation of control muscles did not alter lysomal enzyme activities, denervation of hypertrophying muscles greatly enhanced the activity of these enzymes. Enzymes of energy metabolism were affected to a lesser degree. The results suggest that denervation of hypertrophying muscles causes more extreme changes in muscle weight and lysosomal enzyme activities than denervation alone. The possible implications of this finding are discussed in relation to the rapid atrophy. |
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Keywords: | Energy metabolism Energy enzymes Lysosomal activity Muscle hypertrophy Muscle denervation Muscle atrophy |
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