| 野西瓜总皂苷诱导人肝癌细胞HepG-2凋亡的初步研究 |
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| 引用本文： | 于蕾,崔荣田,莫科,王崴,季宇彬,邹翔. 野西瓜总皂苷诱导人肝癌细胞HepG-2凋亡的初步研究[J]. 天津中医药, 2008, 25(6): 509-511 |
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| 作者姓名： | 于蕾 崔荣田 莫科 王崴 季宇彬 邹翔 |
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| 作者单位： | 1. 北京中医药大学中药学院,北京,100029;哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨,150076;抗肿瘤天然药物教育部工程研究中心,哈尔滨,150076
2. 哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨,150076;抗肿瘤天然药物教育部工程研究中心,哈尔滨,150076 |
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| 基金项目： | 黑龙江省自然科学基金 |
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| 摘 要： | [目的]探讨野西瓜总皂苷(CsS)对人肝癌HepG-2细胞的杀伤作用和诱导凋亡的作用.[方法]四甲基偶氮唑蓝(MTr)法研究CSS对HepG一2细胞的杀伤作用;荧光显微镜观察细胞凋亡形态;碘化吡啶(PI)单染经流式细胞仪检测CSS对HepG-2细胞周期及凋亡的影响.[结果]CSS对HepC-2细胞的生长增殖具有抑制作用;经CSS作用后.HepG-2细胞在出现特征性凋亡形态特征,并出现了G1期阻滞,G2期比例下降,高剂量组出现了G2期消失的现象,50 mg/L剂量作用48 h,凋亡细胞比率高达66.652%.[结论]CSS对人肝癌HepG-2有明显的杀伤和诱导凋亡作用,并出现细胞周期的改变.
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| 关 键 词： | 野西瓜总皂苷 人肝癌HepG-2细胞 细胞凋亡 |
| 收稿时间： | 2008-08-11 |
Preliminary study on apoptosis in HepG-2 of human hepatoma cell line induced by CSS |
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| Yu Lei,Cui Rong-tian,Mo Ke. Preliminary study on apoptosis in HepG-2 of human hepatoma cell line induced by CSS[J]. Tianjin Journal of Traditional Chin Medicine, 2008, 25(6): 509-511 |
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| Authors: | Yu Lei Cui Rong-tian Mo Ke |
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| Affiliation: | Yu Lei1,2,3,Cui Rong-tian2,Mo Ke2,et al (1. The Chinese Pharmacy College of Beijing University of TCM,Beijing 100102,China,2. Harbin University of Commerce,Harbin 150076,3. Engineering Research Center of Natural Anti-cancer Drugs,Ministry of Education,China) |
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| Abstract: | [Objective] To discuss the killing and apoptosis-inducing effect of total alkaloid in the CSS (Capparis spinosa L. saponin, CSS) on human heptocarcinoma cell Line HepG-2. [Methods] The killing effect of the CSS on cell Line HepG-2 was observed by MTT method. Morphological observation of the HepG-2 cells was completed by fluorescence microscope. This test was signed to observe the changes of the cell cycle of HepG-2 affected by the CSS with PI single-staining. [Results] CSS had inhibiting effect on the proli... |
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| Keywords: | CSS Human Heptocarcinoma Cell Line HepG-2 Apoptosis cell cycle change |
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