| 应用DNA体外PCR扩增技术对地贫症进行产前基因诊断 |
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| 引用本文： | 张树球,黄运忠,钟鸣,罗艳红,卢彩珍,李民,陈惠勤,韦红卫,王利民,秦明丽,张慕洁.应用DNA体外PCR扩增技术对地贫症进行产前基因诊断[J].右江民族医学院学报,1990(3). |
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| 作者姓名： | 张树球 黄运忠 钟鸣 罗艳红 卢彩珍 李民 陈惠勤 韦红卫 王利民 秦明丽 张慕洁 |
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| 作者单位： | 右江民族医学院生化教研室，右江民族医学院生化教研室，右江民族医学院生化教研室，右江民族医学院生化教研室，右江民族医学院生化教研室，右江民族医学院生化教研室，右江民族医学院妇产科教研室，右江民族医学院妇产科教研室，右江民族医学院妇产科教研室，右江民族医学院妇产科教研室，右江民族医学院儿科教研室 |
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| 摘 要： | 应用基因工程技术中最新的方法—DNA体外PCR扩增技术,对4例正常人和4例地贫患者的胎儿绒毛DNA中的α和β珠蛋白基因片段进行扩增成功,并对一例Hb Bart’s水肿胎儿进行了基因诊断。本法最大优点为迅速、灵敏、简便,且不需同位素标记探针,适用于边远地区进行地贫症产前基因诊断。
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| 关 键 词： | 基因 脱氧核糖核酸聚合酶类 地中海贫血 |
THE APPLICATION OF DNA AMPLIFICATION IN VITRO WITH POLYMERASE CHAIN REACTION TO THE PRENATAL GENE DIAGNOSIS OF THALASSEMIA |
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| zhang shuqiu.THE APPLICATION OF DNA AMPLIFICATION IN VITRO WITH POLYMERASE CHAIN REACTION TO THE PRENATAL GENE DIAGNOSIS OF THALASSEMIA[J].Journal of Youjiang Medical College For Nationalities,1990(3). |
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| Authors: | zhang shuqiu |
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| Institution: | zhang shuqiu,Department of Biochemistry,etc. Youjiang Medical College for National Minorities,Bose,Guangxi,China |
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| Abstract: | We are successfully applied the recent method of gene enginering technique--DNA amplification in vitro with polymerase chain reaction to the gene piece of the α-and β-globin in fetal chorial villus DNA of 4 normal persons and 4 cases of thalassemia. The gene diagnosis for a hydropic fetus of Hb Bart 's was made. This method does not require isotope labelling probe. It is a rapid, highly sensitive and simple method which is provides for the prenatal gene diagnosis of the patient with thalassemia on the mountainous area. |
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| Keywords: | genes DNA polymerases thalassemia |
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