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雷公藤多甙对急性移植物抗宿主病时小鼠T细胞及其亚群的影响
引用本文:于艳秋,张海鹏,杜烨玮,段忠辉.雷公藤多甙对急性移植物抗宿主病时小鼠T细胞及其亚群的影响[J].中国中西医结合杂志,2002,22(10):770-772.
作者姓名:于艳秋  张海鹏  杜烨玮  段忠辉
作者单位:中国医科大学病理生理教研室,沈阳,110001
基金项目:国家中医药管理局青年基金资助项目 (No .2 0 0 0 -J-Q - 0 6)
摘    要:目的:观察雷公藤多甙对急性移植物抗宿主病时小鼠脾,皮肤,肺,肠组织中T细胞及其亚群(CD3^+,CD4^+,CD8^+细胞)的影响,探讨雷公藤多甙对急性移植物抗宿主病的作用及机理。方法:用直线加速器排空受鼠骨髓后注入供鼠骨髓与脾淋巴细胞的混合液,复制出急性移植物抗宿主病(aGVHD)模型。观察雷公藤多甙,雷公藤多甙加环孢素A(CsA),CsA加甲氨蝶呤对各组小鼠脾CD3^+,CD4^+,CD8^+细胞(流式细胞仪检测),皮肤,肺,肠组织CD3^+,CD4^+,CD8^+细胞浸润情况(应用免疫组化技术检测)的影响。结果:雷公藤多甙组小鼠脾,皮肤,肺组织CD3^+,CD4^+,CD8^+细胞明显低于异基因移植组(P<0.05),而小肠组织CD3^+,CD4^+,CD8^+细胞变化不明显(P>0.05)。结论:雷公藤多甙可降低脾T细胞及亚群数量,减少皮肤肺的T细胞浸润,具有明显的抗aGVHD作用。

关 键 词:移植物抗宿主病  雷公藤多甙  T淋巴细胞
修稿时间:2001年12月26

Effect of Glucosidorum Tripterygii Tororum on T-Lymphocyte and Its Subsets in Mice with Acute Graft-Versus-Host Disease
Authors:YU Yan qiu  ZHANG Hai peng  DU Ye wei
Abstract:Objective: To observe the effect of Glucosidorum tripterygii tororum (GTT) on T lymphocyte and its subsets in mice with acute graft versus host disease (aGVHD). Methods: The recipient mice, after their bone marrow had been evacuated by radiation with linear accelerator, were given the mixture of hone marrow and splenic T lymphocyte obtained from donor mice to duplicate aGVHD model. The effect of GTT, GTT+Cyclosporin A and CsA+Methotrexate on T lymphocyte subsets (CD 3 +, CD 4 + and CD 8 +) in spleen was determined by flow cytometer, and those on lymphocyte infiltration in skin, lung and intestine was detected with immunohistological technique. Results: CD 3 +, CD 4 + and CD 8 + cells in spleen, skin and lung in mice of the GTT treated groups were significantly lower than those in the model group (P<0 05), while in intestinal tissue, the difference was insignificant (P>0 05). Conclusion: GTT could reduce the T lymphocyte and its subsets in spleen and the T lymphocyte infiltration in skin and lung, showing evident anti aGVHD effect.
Keywords:graft  versus  host disease  Glucosidorum tripterygii tororum  T  lymphocyte
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