The preparation of stable phosphorylcholine-erythrocyte conjugates for use in plaque assays |
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| Authors: | T J Lindsay W Birmingham A M Munster R A Winchurch |
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| Affiliation: | Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, U.S.A. |
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| Abstract: | A method for the preparation of stable phosphorylcholine-conjugated erythrocytes is described. This method employs the formation of active esters of phosphorylcholine hydroxyphenylacetic acid (PC-HPA) and results in coupled red cells which are completely stable for 2-3 weeks. Using this procedure up to 80% of the activity was demonstrable after 6 weeks. Cells coupled with PC-HPA are suitable for the demonstration of anti-idiotype effects in a plaquing assay. |
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| Keywords: | antibodies to phosphorylcholine plaque assay idiotypes |
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