抗环瓜氨酸肽抗体在类风湿关节炎中的应用

目的类风湿关节炎(RA)是一种致畸性较高的自身免疫性疾病,本实验探讨抗环瓜氨酸肽抗体(抗-CCP)和类风湿因子(RF)对RA的诊断作用。方法用酶联免疫吸附试验法测定抗-CCP,用速率散射免疫比浊检测RF。结果 380例RA患者抗-CCP和RF阳性率分别为63.4%、68.2%;152例非RA患者抗-CCP和RF阳性率2.0%、15.1%;抗-CCP和RF对RA诊断的灵敏度分别为63.4%、68.9%,特异性分别为98%、84.9%,二者联合时特异性达到97.4%。结论抗-CCP在RA诊断中有良好的灵敏度和特异性,二者联合能极大地提高RA诊断的准确性。

Application of anti-CCP antibody detection in rheumatoid arthritis

Objective To explore the clinical value of anti-cyclic citrullinated peptides（anti-CCP） antibody and rheumatoid factor（RF） in the diagnosis of rheumatoid arthritis（RA）.Methods ELISA was used to measure anti-CCP antibody and the rate scatting immunoturbidimetry assay was adopted to measure RF.Results The positive rates of anti-CCP antibody and RF were 63.4% and 68.2% in 380 RA patients,respectively.The positive rates of anti-CCP antibody and RF were 63.4% and 68.2% in 152 non RA patients,respectively.The sensitivity of anti CCP antibody and RF for diagnosing RA was 63.4% and 68.2%,respectively.The specificity of anti CCP antibody and RF for diagnosing RA was 98% and 84.9%,respectively.The specificity of combined anti-CCP antibody and RF for diagnosing RA was 98.9%.Conclusion The sensitivity and specificity of anti-CCP antibody are satisfactory in diagnosis of RA.Combined detection of anti-CCP antibody and RF greatly improves the accuracy of RA diagnosis.