PCNA的过度表达在宫颈鳞癌诊断中的临床意义

目的　通过对 45例宫颈鳞癌中PCNA基因过度表达的研究 ,探讨其在子宫颈鳞形细胞癌诊断中的临床意义。方法　标本采用常规石蜡切片、HE染色及ABC法免疫组化染色 ,光镜观察 ,并对增殖细胞核抗原指数 (PCNALI)进行计数。结果　从正常宫颈鳞状上皮到CINⅡ级到子宫颈鳞癌 ,表达PCNA阳性的细胞范围和阳性强度呈明显增高的趋势 ,三者间PCNALI差异有显著性 (P <0 .0 5 )。在 45例鳞状细胞癌中PCNALI随着肿瘤分级增高而增高。鳞癌Ⅰ级、Ⅱ级和Ⅲ级之间PCNALI差异有显著性 (P <0 .0 5 )。局部淋巴结有转移癌者和无转移癌者PCNALI差异亦有显著性 (P <0 .0 5 )。结论　PCNALI对于检测宫颈鳞癌的发生与发展、早期诊断及判断肿瘤的生物学行为和预测患者的预后等方面具有重要的意义。

The overexpression of PCNA in the diagnosis of cervical squamous carcinoma

Objective To study the clinical significance of the overexpression of proliferative cell nuclear antigenic (PCNA) gene in the diagnosis of squamous cell carcinoma of cervix in 45 patients with cervical squamous carcinoma. Methods The paraffin slides were cut and stained with HE, and immunohistochemically stained with ABC method. Then the PCNALI (PCNA/ cyclin labeling index). was counted under optical microscope. Results From normal cervical squamous epithelium to CINⅡ (Cervical intraepithelial neoplasiaⅡ)and to squamous cell carcinoma of cervix, the scale of cells which express the positive PCNA and the positive intensity tended to increase, and the differences of PCNALI between the mentioned above three were statistically significant (P<0.05). In 45 patients with squamous cell carcinoma, the PCNALI increased as the increasing of the tumor grading. The differences of PCNALI between saquamous carcinomaⅠ,Ⅱand Ⅲ were statistically significant (P<0.05). The differences of PCNALI were also significant between patients with metastatic carcinoma of regional lymph nodes and those without metastatic carcinoma. (P<0.05). Conclusions PCNALI played an important role in monitoring the occurrence and development of cervical squamous carcinoma, early diagnosing and judging the biologic behavior of neoplasm and also in predicting the prognosis of the patients.