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| X刀治疗大鼠C6脑胶质瘤诱发凋亡及抑癌基因p16蛋白的表达 | |
| 引用本文： | 何跃,袁先厚,王伦长,涂汉军,黄宽明,李新建,秦军,王辉.X刀治疗大鼠C6脑胶质瘤诱发凋亡及抑癌基因p16蛋白的表达[J].肿瘤防治研究,2003,30(5):357-358,412,F002. |
| 作者姓名： | 何跃 袁先厚 王伦长 涂汉军 黄宽明 李新建 秦军 王辉 |
| 作者单位： | 1. 郧阳医学院附属太和医院神经外科 2. 武汉大学中南医院神经外科 3. 十堰市太和医院神经外科 |
| 基金项目： | 湖北省科技厅资助项目 (2 0 0 2A0 30 0 5 ) |
| 摘 要： | 目的 为了探讨经X刀照射诱导C6脑胶质瘤凋亡及其抑癌基因p16蛋白的表达。方法 建立大鼠C6脑胶质瘤模型 ,经X刀照射后 ,采用TUNEL法检测细胞凋亡 ,免疫组化SABC方法检测抑癌基因p16蛋白表达的变化。结果 治疗组凋亡阳性细胞数明显高于对照组 (P <0 .0 5 ) ;p16基因在蛋白水平表达率也高于对照组 (P <0 .0 5 )。结论 X线可诱导C6脑胶质瘤细胞发生凋亡 ,抑癌基因 p16在辐射诱导凋亡中起重要的作用。 |
| 关 键 词： | 放射外科 脑胶质瘤 凋亡 抑癌基因 p16 |
| 文章编号： | 1000-8578(2003)05-0357-02 |
| 收稿时间： | 2003-3-24 |
| 修稿时间： | 2003-4-8 |
The induced apoptosis and the p16 Gene's expression in treating rat C6 glioma with X-knife |
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| HE Yue,YUAN Xian-hou,WANG Lun-chang,et al.The induced apoptosis and the p16 Gene''''s expression in treating rat C6 glioma with X-knife[J].Cancer Research on Prevention and Treatment,2003,30(5):357-358,412,F002. | |
| Authors: | HE Yue YUAN Xian-hou WANG Lun-chang |
| Institution: | HE Yue,YUAN Xian-hou,WANG Lun-chang,et al Department of Neurosurgery,The Affiliated Hospital of Yunyang Medical University,Shiyan 442000,China |
| Abstract: | Objective To investigate the apoptosis of C6 glioma cell and the p16 gene's protein expression after X-knife radiation. Methods By establishing a rat C6 glioma model,after X-knife, TUNEL and immunohistochemistry technique (SABC methods) was employed to inspect the apoptotic cells and the p16 gene's protein expression respectively. Results X-radiation can induce apoptosis,the apoptosis and the p16 gene's protein expression in the treatment group is higher than that in control group ( P < 0.05 ). Conclusion X-radiation can induce apoptosis in the rat C6 glioma cells, the tumor suppressor gene p16 play an important role in radiation-induced apoptosis. |
| Keywords: | Radio surgery Glioma Apoptosis Tumor suppressor gene p16 |
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