原发性开角型青光眼患者房水中IL-37和IL-6的表达及意义

目的:检测原发性开角型青光眼(POAG)患者房水中IL-37和IL-6的含量,并分析IL-6和IL-37含量与眼压或视野平均缺损的相关性。方法:采用前瞻性病例对照研究,利用酶联免疫吸附试验(ELISA)对2019-06/2020-01在南京医科大学附属眼科医院连续就诊的25例POAG患者和25例年龄相关性白内障(ARC)患者房水中IL-37和IL-6含量进行检测。同时测定POAG组患者眼压、视野平均缺损。结果:房水中IL-37和IL-6含量POAG组患者为25.80±2.87、43.87±7.75pg/mL,ARC组患者为23.75±3.88、36.53±7.60pg/mL,两组间IL-37和IL-6含量比较均有差异(P<0.05)。IL-6与眼压正相关(r=0.5817,P<0.05),IL-37与视野缺损正相关(r=0.4520,P<0.05)。结论:POAG患者房水中IL-37、IL-6含量均显著高于ARC组。IL-37和IL-6介导的免疫炎症反应可能参与了POAG的发病机制。

Expression and significance of IL-37 and IL-6 in aqueous humor of patients with primary open angle glaucoma

AIM: To test concentrations of IL-37 and IL-6 in aqueous humor samples of patients with primary open angle glaucoma(POAG), and analyze the potential relationship to intraocular pressure(IOP)and mean defect of visual field.

METHODS: Prospective consecutive non-randomized comparative cohort study was conducted. Totally 25 POAG patients and 25 age related cataract(ARC)patients in Nanjing Medical University Eye Hospital from June, 2019 to January, 2020 were collected. The levels of IL-37 and IL-6 in the aqueous humor were detected by enzyme-linked immunosorbent assay(ELISA). The IOP and mean defect of visual field of patients in the POAG group were also measured.

RESULTS: The IL-37 concentrations in aqueous humor of POAG group and control group were 25.80±2.87pg/mL and 23.75±3.88pg/mL respectively(P<0.05). The IL-6 concentrations in aqueous humor of POAG group and control group were 43.87±7.75pg/mL and 36.53±7.60pg/mL respectively(P<0.05). In POAG group, the concentration of IL-37 was significantly positively correlated with mean defect of visual field(r=0.4520, P<0.05), the concentration of IL-6 was significantly positively correlated with IOP(r=0.5817, P<0.05).

CONCLUSION: Significant differences in aqueous humor levels of IL-37 and IL-6 between glaucoma and control patients support the hypothesis that immune inflammation are involved in the pathogenesis of POAG.