水蛭冻干粉对糖尿病肾病大鼠肾组织损伤的保护作用

目的 探讨水蛭冻干粉对糖尿病肾病大鼠肾组织损伤的保护作用.方法 SD大鼠随机选取8只作为对照组,其余大鼠给予高脂饲料喂养联合ip链脲佐菌素(35 mg/kg)建立糖尿病肾病模型,成模大鼠随机分为模型组及水蛭冻干粉低、中、高剂量(0.3、0.6、1.2 g/kg)组.给药16周后,检测大鼠24 h 尿微量白蛋白(24 h...

Protective effects of hirudo lyophilized powder on renal injury in diabetic nephropathy rats

Objective To explore the intervention of hirudo lyophilized powder in the renal tissue injury of diabetic nephropathy rats. Methods Eight SD rats were selected as control group, and the remaining rats were used to establish diabetic nephropathy models by high-fat diet combined with ip streptozotocin(35 mg/kg). Diabetic nephropathy rats were randomly divided into model group, hirudo lyophilized powder low-, medium-, high-dose(0.3, 0.6, 1.2 g/kg) group. After administered with corresponding drug for 16 weeks, the 24 h-urine microalbumin, blood glucose, urea nitrogen(BUN), creatinine(Scr) and KW/BW were detected;The activity of superoxide dismutase(SOD) and level of malondialdehyde(MDA) in serum were measured by TBA and hydroxylamine method respectively;The levels of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), and monocyte chemokine-1(MCP-1) in serum were analyzed by ELISA;The related protein expressions of JAK2/STAT1/STAT3 pathway were detected in renal tissues by western blotting. Results Compared with control group, 24-h urine microalbumin, fasting blood glucose, BUN, Scr, and KW/BW were significantly increased(P < 0.01);The serum levels of MDA, TNF-α, IL-1β, and MCP-1 were significantly increased(P < 0.01);The activiy of SOD was significantly inhibited(P < 0.01);The JAK2/STAT1/STAT3 signaling pathway was activated in renal tissues of model group(P < 0.05, 0.01). Compared with model group, the 24 h urinary microalbumin excretion was reduced(P < 0.01), renal function(BUN, Scr) and KW/BW were significantly improved(P < 0.05, 0.01), the degree of oxidative stress and serum inflammatory factor levels were decreased(P < 0.05, 0.01), and the activation of JAK2/STAT1/STAT3 signaling pathway was inhibited in hirudo lyophilized powder group(P < 0.05, 0.01). Conclusion Hirudo lyophilized powder could inhibit oxidative stress and inflammation injury, inhibit the activation of JAK2/STAT1/STAT3 signaling pathway, reduce urinary microalbumin excretion, and improve renal function, and thus exert renal protective effects.