紫花地丁全草化学成分研究

目的 研究紫花地丁Violayedoensis全草的化学成分.方法 采用硅胶、ODS、SephadexLH-20凝胶和半制备型高效液相等柱色谱方法进行分离纯化,通过理化性质结合NMR、MS等谱学数据分析鉴定化合物结构.结果 从紫花地丁95％乙醇提取物中分离得到21个化合物,分别鉴定为秦皮乙素-6-O-β-D-呋喃芹糖基...

Chemical constituents from whole herbs of Viola yedoensis

Objective To investigate the chemical constituents from whole herbs of Viola yedoensis. Methods The chemical constituents were isolated and purified by silica gel, ODS column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC. The structures of the isolated compounds were identified by physicochemical properties, NMR and MS spectroscopic methods. Results A total of 21 compounds were isolated from the 95% ethanol extract of V. yedoensis, and their structures were identified as aesculetin 6-O-β-D-apiofuranosyl-(1→2)-β-D-glucopyranoside(1), ethyl indole-3-carboxylate(2), dehydrololiolide(3), scoparone(4), taraxacin(5), salicin(6), syringin(7),(E)-4-hydroxy-4-[3′-(β-D-glucopyranosyloxy) butylidene]-3,5,5-trimethyl-2-cyclohexen-l-one(8), citroside A(9),(7 S,8 R)-dihydrodehydrodiconiferyl alcohol-9-O-β-Dglucopyranoside(10), 3-O-α-L-(4-O-acetyl)rhamnopyranosyl kaempferol 7-O-α-L-rhamnopyranoside(11), kaempferol-7-O-α-Lrhamnopyranoside(12), kaempferol-3-O-α-L-rhamnopyranoside(13), kaempferitrin(14), kaempferol-3-O-β-D-glucopyranoside(15), apigenin(16),(+)-isololiolide(17), uridine(18), β-adenosine(19), 6-hydroxycoumarin-7-O-α-L-rhamnosyl-(1→6)-O-β-D-glucoside(20), and(7 S,8 R)-dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside(21). Conclusion Compound 1 is obtained as a new compound, named as vioyegluapiside and compound 2 as a new natural product, with compounds 5-11 and 18 isolating from the genus Viola for the first time and with compounds 12 and 21 from plant V. yedoensis for the first time.