EGFR在高能电子线大鼠皮肤辐射损伤模型中的表达和意义

目的　探讨辐射对皮肤表皮生长因子受体（EGFR）表达的影响。方法　采用免疫组化链霉亲和素─卵白素─生物素复合物（SABC）法检测40例大鼠皮肤辐射损伤模型标本EGFR的表达,实验动物按照射剂量分为4个剂量组。结果　5Gy组、15Gy组、30Gy组、45Gy组EGFR阳性表达率分别为27.0%、49.3%、72.2%、87.6%，与正常对照组(10.8%)比较，均有显著差异（均P＜0.01）。另外，各照射组间差异亦显著（P＜0.01），表现为随着照射剂量的增加,EGFR阳性表达率亦增加。结论　辐射可显著增加皮肤组织中EGFR的表达，可能为辐射激活并扩增c-erbB-1基因的结果；并在一定照射剂量范围内，随着照射剂量的增加，c-erbB-1基因的激活与扩增增强，这可能成为皮肤溃疡难以愈合的重要因素之一。

Expression andSignificance of EGFR Protein in Model of Radiation Injury in Mouse Skin

Objective The expression of EGFR protein was studied by SABC immunohistochemistry in 40 cases of model of radiation injury in mouse skin.Methods Experiment animals were divided into four groups according to radiation dose.Results The positive rates were 27.0%,49.3%,72.2%,87.6% in 5 Gy group,15 Gy group,30 Gy group,45 Gy group respectively , showing significant difference ( P <0.01 ).While the positive rate was 10.8% in normal control group , with significant difference ( P <0.01) compared with each radiation group .Conclusion The enhancement of expression of EGFR in accordance with the increase of radiation dose in certain dose range might be one important factor related to c-erbB-1 gene activated and enlarged by radiation, and the overexpression of EGFR protein might be related to poor healing in radiation skin injury.