腺苷蛋氨酸治疗新生大鼠高未结合胆红素血症作用机制的研究

目的:探讨腺苷蛋氨酸治疗新生大鼠高未结合胆红素血症(HUB)的作用机制。方法:95只新生SD大鼠随机分为模型对照组、治疗对照组和实验组,皮下注射盐酸苯肼建立HUB模型,建模同时3组分别腹腔注射等量生理盐水、苯巴比妥/尼可刹米和腺苷蛋氨酸,每天1次,共7d。不同时间采血测定3组大鼠血清胆红素水平及肝脏胆红素-尿苷二磷酸葡糖醛酸基转移酶(BUGT)活性。结果:与同期模型对照组比较,治疗对照组血清未结合胆红素水平更低,BUGT活性更高(P<0.05或P<0.01);与同期模型对照组和治疗对照组比较,实验组血清未结合胆红素及结合胆红素水平显著更低,BUGT活性更高(P<0.05或P<0.01)。结论:腺苷蛋氨酸可通过升高肝脏BUGT活性,降低血清未结合胆红素及结合胆红素水平,有效治疗新生大鼠HUB。

Mechanism of Ademetionine for Treating Hyper - unconjugated Bilirubinemia in Neonate Rats

OBJECTIVE： To investigate the efficacy and mechanism of ademetionine for treating hyper - unconjugated bilirubinemia in neonate rats. METHODS： The model of hyper-unconjugated bilirubinemia was established in 95 neonate SD rats by subcutaneously injection of phenylhydrazine hydrochloride, then the rats were randomly assigned to model control group （treated with normal saline）, therapeutic control group （phenobarbital/nikethamide） and the therapeutic group （s- adenosyl - 1- methionine） q .d for 7 days all by intraperitoneal injection. Blood samples were taken at different time for the analysis of the hepatic BUGT activity and serum bilirubin. RESULTS： In therapeutic control group compared with the model control group, the serum unconjugated bilirubin was lower, and the hepatic BUGT activity of therapeutic was higher（P 〈 0.05 or P 〈 0.01）. In therapeutic group compared with the model control group or therapeutic control group, the serum unconjugated bilirubin and conjugated bilirubin were significantly lower and the hepatic BUGT activity was higher（P 〈 0.05 or P 〈0.01） . CONCLUSION： The mechanism for S- adenosyl- 1- methionine to effectively treat hyper- unconjugated bilirubinemia in neonate rats is possibly associated to the up - regulation of hepatic BUGT activity and the down - regulation of the unconjugated and conjugated bilirubin in serum.