Hepatitis B Virus Genetic Typing Using Mass-Spectrometry |
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Authors: | M. V. Malakhova E. N. Ilina V. M. Govorun S. A. Shutko K. R. Dudina O. O. Znoyko E. A. Klimova N. D. Iushchuk |
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Affiliation: | (1) Scientific Research Institute of Physical-Chemical Medicine, Moscow, Russia;(2) Moscow State Medical-Stomatological University, Moscow, Russia |
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Abstract: | Mini-sequencing with subsequent result registration using MALDI-ToF mass-spectrometry was employed for hepatitis B virus genetic typing in Russian population. This approach was employed for hepatitis B virus genetic typing in HBsAg-positive patients with chronic hepatitis B, hepatitis of combined etiology and hepatic cirrhosis and allowed to show the prevalence of D genotype (83.3%) in all groups of patients. Other hepatitis B virus genotypes: genotype A (5.9%), genotype C (3.6%), and mixed infection with D and C (7.2%) were also found in patients with chronic hepatitis B and hepatic cirrhosis. All genotypes were found in patients with chronic hepatitis B and hepatic cirrhosis. Chronic hepatitis of combined etiology was noted only in patients with genotype D. Possibility of detection of mixed infection with hepatitis B viruses of various genotypes is a distinct advantage of mini-sequencing approach over direct nucleotide sequence evaluation for hepatitis B virus genetic typing. Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 147, No. 2, pp. 181-186, February, 2009 |
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Keywords: | viral hepatitis B genetic typing mini-sequencing mass-spectrometry |
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