CR2和MXLIP在低分化鼻咽癌组织中的差异表达

目的 为了解低分化鼻咽癌组织与非癌症组织之间的基因表达差异,进一步找出有明显差异表达的基因作为诊断鼻咽癌新的分子标志.方法 实验通过14 112个点的cDNA基因表达谱芯片,比较20例低分化鼻咽癌组织和15例鼻咽慢性炎症组织之间的表达差异,并通过荧光实时定量PCR对50例鼻咽癌组织进行验证.结果 基因芯片的分析结果显示,9个基因（q〈0.01）至少在17例（85%）鼻咽癌组织中有2倍差异表达,包括8个下调基因（TAOK3,SLC16A2,PRB4,AMY2B,B3GALT4,MSMB,RPS27,CR2）和1个上调基因（MXLIP）.进一步研究表明,选用CR2和MXLIP对50例低分化鼻咽癌组织荧光实时定量PCR分析与3例鼻咽慢性炎症组织进行比较,与芯片实验结果相同,CR2在41例（82%）鼻咽癌组织中下调,MXLIP在42例（84%）鼻咽癌组织中上调.结论 我们认为这两个基因可以作为诊断低分化鼻咽癌新的标志基因.

The differential expression of CR2 and MXLIP in poor differentiation nasopharyngeal carcinoma

Objective To investigate the alteration of gene expression profiles between nasopharyngeal carcinoma （NPC） and non-neoplasm tissues then identified significant differentially expressed genes from NPC as novel markers. Methods In our study, cDNA microarrays, which contain 14 112 points of full length human genes, in 20 NPC patients and in 15 nasopharyngeal chronic phlogistic tissues were used for revealing some new differentially expressed genes and molecular markers. Further, real time PCR was used to validate results of 2 groups in additional 50 poorly differentiated NPC tissues. Results Nine significant differential expression genes （ q 〈 0. 01 ）, with 2-fold or more alteration expression, were found differential expression in more than 17 （85 % ） specimens. Of them, 8 genes were over-expression （ TAOK3, SLC16A2, PRB4, AMY2B, B3 GALT4, MSMB, RPS27, CR2 ） were and under-expression （MXLIP） and 1 gene was up-expression. For further research, CR2 and MXLIP expression were detected by real time PCR in additional 50 NPC specimens. Compared with 3 nasopharyngeal chronic phlogistic tissues, CR2 was lower expression in 41 （82%） specimens and MXLIP was over expression in 42 （84%） specimens. This result was consistent with our microarray data. Conclusion These two differentially expressed genes could be used as novel markers for poor differentiation NPC.