HPLC法测定四维钙片中维生素C、B1、B2的含量

目的：建立高效液相色谱法测定四维钙片中维生素C、维生素B1和维生素B2含量的方法.方法：采用SHISEIDOC18（250 mm×4.6 mm,5μm）色谱柱,以0.05％磷酸溶液（含0.005 mol·L-1己烷磺酸钠）-乙腈（88：12）为流动相,流速：1.0ml· min-1,检测波长：244 nm,柱温：30℃,进样量：20μm.结果：维生素C、维生素B1和维生素B2的线性范围分别为7.52～90.24 μg· ml-1（r=1.000 0）,1.32 ～15.82 μg·ml-1（r=1.000 0）和1.27～15.24μg·ml-1 （r=1.000 0）;平均回收率分别为101.63％、99.77％和100.25％,RSD分别为1.51％、1.48％和1.84％（n=9）.结论：本法操作简便,结果准确、可靠,可用于四维钙片中维生素C、B1、B2的含量测定.

Determination of Vitamin C, Vitamin B1 and Vitamin B2 in Siweigai Tablets by HPLC

Objective： To establish an HPLC method for the determination of vitamin C, vitamin BI and vitamin B2 in Siweigai tab- lets. Methods： A SHISEIDO Cls column （ 250 mm x 4.6 mm,5 txm） was used for the separation with 0.05 % phosphoric acid （ contai- ning 0.005 mol · L-1 sodium hexanesulfonate）-aeetonitrile （88： 12）as the mobile phase, and the flow rate was 1.0 ml · min-1. The detection wavelength was 244nm, the column temperature was 30℃ and the injection volume was 20 μl. Results： The linear ranges of vitamin C, vitamin B1 and vitamin B2 was 7.52-90.24 μg· ml-1（ r = 1. 000 0）, 1.32-15.82 μg. ml-1（ r = 1. 000 0） and 1.27- 15.24 μg- ml-1 （r = 1.000 0）, respectively. The average recovery was 101.63% , 99.77% and 100.25% with RSD of 1.51%, 1.48% and 1.84% （ n = 9 ） , respectively. Conclusion： The method is simple and accurate in the analysis of Siweigai tablets.