芒果苷对大鼠心肌缺血再灌注损伤的保护作用

目的探讨芒果苷对大鼠心肌缺血再灌注损伤的保护作用及机制。方法48只SD大鼠随机分为6组:假手术组、缺血再灌注模型组、芒果苷10mg/kg、20mg/kg和40mg/kg剂量组、地奥心血康组,分别给予相应剂量芒果苷、地奥心血康或等容量的生理盐水灌服21d,末次给药后1h采用左冠状动脉前降支结扎40min再灌120min建立在体大鼠心肌缺血再灌注模型。假手术组完成模型全部操作只穿线不结扎。电镜观察心肌超微结构改变,测定心肌组织髓过氧化物酶,观察中性粒细胞浸润;检测血清乳酸脱氢酶活性;分别检测心肌组织超氧化物歧化酶和丙二醛含量。结果芒果苷可以改善心肌缺血再灌注大鼠心肌超微结构,芒果苷小剂量组就能使超氧化物歧化酶活力升高,心肌组织髓过氧化物酶、丙二醛、乳酸脱氢酶含量降低。结论芒果苷对缺血再灌注损伤的心肌具有保护作用,其机制可能与提高超氧化物歧化酶活性,增强心肌抗氧化能力,减轻氧自由基损伤,稳定细胞膜,减轻心肌组织中性粒细胞浸润有关。

Cardioprotective Effects of Mangiferin on Myocardial in Schemia Reperfusion Injury in Rats

Aim To study the effect and possible mechanism of mangiferin on myocardial ischemia reperfusion (MIR) injury in rats. Methods Fouty-eight SD rats were randomly divided into six groups: sham group, model group, mangiferin groups of 10 mg/kg, 20 mg/kg, 40 mg/kg and Diaoxinxuekang group. Each group were injected with corresponding concentrations of mangferin, Diaoxin xuekang or equal volume of saline gastrogavaged for 21 d. 0ne hour after the last administration myocardial ischemia reperfusion models were obtained by ligated left anterior descending coronary artery 40 minutes and followed by 120 minutes reperfusion and sham group was given all the procedures except ligation. Ultrastructure of myocardium with TEM and infiltration of polymorpho nuclear neutrophils (PMN) in myocardium with myeloperoxidase (MPO) were observed; serum activity of lactate dehyd -rogease (LDH), myocardial activity of superox -ide dismutase (SOD) and contents of malondialdehyde (MDA) were detected respectively. Results Mangiferin could improve myocardial pathology and ultrastructurein mangiferin 10 mg/kg group the activities of SOD was higher (P<0.05); the contents of MPO, MDA, LDH were significantly lower (P<0.05). Conclusion Mangiferin can protect MIR. The myocardial protective mechanism of it may be realized by enhancing the activation of SOD activity,enhancing myocardial antioxygen capability, stabilizing myocardial cellular membrance and alleviating infiltration of polymerrphonuclear neutrophils(PMN) in myocardium.