| 用固相聚合酶链反应检测恶性疟原虫DNA特异片段 |
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| 引用本文： | 于洋 何建文. 用固相聚合酶链反应检测恶性疟原虫DNA特异片段[J]. 中国寄生虫学与寄生虫病杂志, 1994, 12(2): 122-124 |
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| 作者姓名： | 于洋 何建文 |
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| 作者单位： | 上海第二军医大学长海医院实验诊断科 |
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| 摘 要： | 本文建立了固相聚合酶链反应（ＰＣＲ）技术检测恶性疟原虫的方法，并对培养的ＦＣＣｌ／ＨＮ株及西双版纳恶性疟患者进行检测，结果表明该法对ＦＣＣｌ／ＨＮ株ＤＮＡ敏感到０．２ｐｇ，相当于１０个疟原虫，对西双版纳疟疾患者检测恶性疟３０例，间日疟４例，证实ＰＣＲ仅特异扩增恶性疟原虫ＤＮＡ。提示固相ＰＣＲ是早期、敏感、特异检测恶性疟原虫的方法。
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| 关 键 词： | 固相PCR 恶性疟原虫 DNA |
DETECTION OF SPECIFIC DNA FRAGMENT OF PLASMODIUM FALCIPARUM BY SOLID PHASE PCR |
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| Yu Yang,He Jianwen,Cai Longrong,Ye Fangyun,Xin Hong. DETECTION OF SPECIFIC DNA FRAGMENT OF PLASMODIUM FALCIPARUM BY SOLID PHASE PCR[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1994, 12(2): 122-124 |
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| Authors: | Yu Yang He Jianwen Cai Longrong Ye Fangyun Xin Hong |
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| Affiliation: | Yu Yang,He Jianwen,Cai Longrong,Ye Fangyun,Xin Hong Department of Clinical Laboratory,Changhai Hospital,Second Military Medical University,Shanghai 200433 |
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| Abstract: | Solid phase PCR for detecting Plasmodium falciparum was established and used to detect cultured FCCl/HN isolate and malaria patients in Yunnan Province.The results revealed that the sensitivity of the method for detecting FCCl/HN isolate DNA was as minimal as 0.2 pg,or about 10 parasites. The specificity of the method was confirmed by discriminating malaria patients infected with different species of Plasmodium .The results suggest that solid phase PCR is a specific and sensitive method for early detection of P.falciparum. |
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| Keywords: | Solid phase PCR Plasmodium falciparum DNA |
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