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| 酒精抑制脑星形胶质细胞胶原纤维酸性蛋白和S_(100)蛋白的表达 | |
| 引用本文： | 王霞,屈卫东,张天宝,吴德生,蒋颂辉,朱惠刚. 酒精抑制脑星形胶质细胞胶原纤维酸性蛋白和S_(100)蛋白的表达[J]. 卫生研究, 2006, 35(4): 428-430 |
| 作者姓名： | 王霞 屈卫东 张天宝 吴德生 蒋颂辉 朱惠刚 |
| 作者单位： | 1. 教育部公共卫生安全重点实验室,复旦大学公共卫生学院环境卫生学教研室,上海,200032 2. 第二军医大学卫生毒理学教研室 3. 四川大学华西公共卫生学院环境卫生学教研室 |
| 基金项目： | 国家高技术研究发展计划(863计划);国家自然科学基金;复旦大学校科研和教改项目 |
| 摘 要： | 目的研究酒精对脑星形胶质细胞GFAP和S_(100)蛋白表达的影响。方法对体外分离培养的大鼠胚胎脑星形胶质细胞分别施以不同剂量酒精(2、5和20mmol/L),染毒7天后,以免疫细胞化学法观察酒精对星形胶质细胞GFAP和S_(100)蛋白表达的影响。结果随酒精剂量增加,脑星形胶质细胞GFAP和S_(100)蛋白表达强度下降,高剂量组细胞数量轻度减少。结论提示酒精能抑制星形胶质细胞GFAP和S_(100)蛋白表达。酒精诱导的星形胶质细胞蛋白表达异常可能是酒精引起中枢神经系统发育异常的主要机制之一。 |
| 关 键 词： | 酒精 星形胶质细胞 胶原纤维酸性蛋白 S_(100) |
| 文章编号： | 1000-8020（2006）04-0428-03 |
| 收稿时间： | 2005-09-27 |
| 修稿时间： | 2005-09-27 |
Alcohol inhibited the expression of glial fibral acidic protein and S100 of astrocytes |
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| Wang Xia, Qu Wei-dong, Zhang Tian-bao, Wu De-sheng,et al.. Alcohol inhibited the expression of glial fibral acidic protein and S100 of astrocytes[J]. Journal of hygiene research, 2006, 35(4): 428-430 | |
| Authors: | Wang Xia Qu Wei-dong Zhang Tian-bao Wu De-sheng et al. |
| Affiliation: | Key Laboratory of Public Health Safety, Ministry of Education, Department of Environmental Health, School of Public Health, Fudan University, Shanghai 200032, China |
| Abstract: | Objective To explore effects of alcohol on the expression of gial fibral acidic protein(GFAP) and S_(100) of astrocytes in brain.Methods Different dosages of alcohol (2,5 and 20mmol/L) were administered to astrocytes of fetal rat brain in vitro. The expression of GFAP and S_(100) of astrocytes was detected by immunocytochemistry, respectively. Results With the dosage of alcohol increasing, the expression of GFAP and S_(100) was reduced and the number of astrocytes in high dosage group was observed to be decreased slightly.Conclusion It suggested that alcohol could repress the expression of GFAP and S_(100) of astrocytes, which might be the main mechanism of developmental abnormalities of central nervous system induced by alcohol. |
| Keywords: | S100 |
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