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The enigmatic role of cholinergic reflex in the pathogenesis of Chagas disease |
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| Authors: | Luiz G. F. de A. B. D’Elia Zanella Agnaldo Bruno Chies Maria Angélica Spadella Altino Luiz Silva Therezo Patrícia de Souza Rossignoli Fernando Frei Luciamáre Perinetti Alves Martins |
| Affiliation: | 1. Department of Parasitology, Marília Medical School, Monte Carmelo avenue, 800, Fragata, 17519-100, Marília, SP, Brazil 2. Department of Pharmacology, Marília Medical School, Marília, SP, Brazil 3. Department of Embryology, Marília Medical School, Marília, SP, Brazil 4. Department of Pathology, Marília Medical School, Marília, SP, Brazil 5. Department of Physiotherapy and Occupational Therapy, S?o Paulo State University, Marília, SP, Brazil 6. Department of Biology, State University of S?o Paulo, Assis, SP, Brazil |
| Abstract: | This study evaluated the inflammatory process in the colons of mice infected with Trypanosoma cruzi QM2 strain, through the analysis of muscle reactivity and the measurement of butyrylcholinesterase (BuChE) in plasma. “Swiss” mice were infected with T. cruzi QM2 strain and after 15 (G15), 30 (G30), 60 (G60), 90 (G90), and 210 (G210) days, each group had blood collected for the measurement of butyrylcholinesterase plasma concentrations ([BuChE]), a measure which functioned as an indicator of plasmatic Ach levels. All groups, except G15, had a segment of proximal colon removed to assess muscle reactivity to acetylcholine (Ach) and noradrenaline (NA) stimulation. After reactivity tests, the tissues were then fixed and stained with hematoxylin and eosin (HE) for histological evaluation of inflammatory response. The QM2 strain did induce inflammatory process in mice colon, and demonstrated differences in muscular contraction between the G60 and G210 groups, with p?p?T. cruzi infection. |
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