喷砂酸蚀与双重酸蚀钛表面对成骨细胞行为影响的对比研究

目的 对比研究喷砂酸蚀与双重酸蚀钛表面对成骨细胞生物学行为的影响。 方法 在纯钛试件表面分别进行喷砂酸蚀与双重酸蚀处理。以光滑钛表面（Ti）为对照组，喷砂酸蚀钛表面（Ti-SLA）、双重酸蚀钛表面（Ti-DA）为实验组，通过扫描电镜（SEM）、表面接触角测试、X射线光电子能谱（XPS）观察分析三种钛表面的微形貌、润湿性和元素组成。将MC3T3-E1成骨细胞接种于三组试件表面，研究不同钛表面对成骨细胞生物学行为的影响。 结果 SEM观察显示Ti-DA组试件表面形成了较Ti-SLA组更均匀细密的微米级凹坑结构；各组试件的表面接触角无显著差异；XPS分析显示Ti-SLA组试件表面有微量铝元素残留；成骨细胞在三组试件表面的粘附、增殖无显著差异，而Ti-DA组显著促进成骨细胞的分化。 结论 与喷砂酸蚀钛表面相比，双重酸蚀钛表面的微米级凹坑结构更均匀细密，且无铝元素残留，能更有效地促进成骨细胞分化。

A comparative study of biological behaviors of osteoblasts on SLA and double acid etched titanium surfaces

Objective To comparatively evaluate the influences of sandblasting acid etched (SLA) titanium surface and double acid etched titanium surface on biological behaviors of osteoblasts. Methods Sandblasting acid-etching and double acid-etching were performed respectively on pure titanium surfaces. The pure titanium surface (Ti) was used as the control group; sandblasting acid etched surface (Ti-SLA) and double acid etched surface (Ti-DA) were used as two experiment groups. The micro-topography, wettability and elemental compositions of different titanium surfaces were observed and analyzed via scanning electron microscopy (SEM), contact angel test and X-ray photoelectron spectroscopy (XPS). MC3T3-E1 cells were seeded on the surface of samples of three groups to investigate the effects of different titanium surfaces on biological behaviors of osteoblasts. Results SEM observation showed that more uniform dense micro-porous structure formed on the Ti-DA surface compared with the Ti-SLA surface. There were no evident differences in surface contact angles among three different groups. XPS analysis revealed that trace aluminum element had remained on the Ti-SLA surface. Adhesion and proliferation abilities of osteoblasts on the surface of samples of three groups showed no obvious differences, while Ti-DA group significantly promoted osteoblast differentiation. Conclusion Compared with the SLA titanium surface, the double acid etched titanium surface exhibits more uniform dense micro-topography without aluminum residue, and effectively promotes osteoblast differentiation.