| 鼠醛糖还原酶的分离纯化和性质分析 |
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| 引用本文： | 王家富,董砚虎,刘长山.鼠醛糖还原酶的分离纯化和性质分析[J].潍坊医学院学报,1996,18(1):15-18. |
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| 作者姓名： | 王家富 董砚虎 刘长山 |
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| 摘 要： | 利用离心、盐析及多步层析法将鼠睾丸醛糖还原酶（ａｌｄｏｓｅｒｅｄｕｃｔａｓｅ，ＡＲ）分离纯化，纯化的酶经ＳＤＳ－ＰＡＧＥ电泳鉴定示一单一蛋白条带，ＡＲ分子量为３９０００±１０００，ＡＲ底物特异性，按１／Ｋｍ大小依次排列为：Ｐ－硝基苯醛，ＤＬ－甘油醛，Ｄ－葡萄糖醛酸，Ｄ－木糖，Ｄ－半乳糖，Ｄ－葡萄糖。
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| 关 键 词： | 醛糖还原酶 睾丸 纯化 糖尿病 |
PURIFICATION AND CHARACTERIZATION OF ALDOSE REDUCTASE FROM RAT TESTIS |
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| Wang Jiafu,Dong Yanhu,Liu Changshan, et al.PURIFICATION AND CHARACTERIZATION OF ALDOSE REDUCTASE FROM RAT TESTIS[J].Journal of Weifang Medical College,1996,18(1):15-18. |
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| Authors: | Wang Jiafu Dong Yanhu Liu Changshan |
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| Institution: | Institute of Endocrinology 261042 |
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| Abstract: | |
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| Keywords: | aldose reductase testis purification diabetes |
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