miR-15a与临床膀胱癌患者术后复发的相关性分析

目的 探讨miR-15a表达水平在膀胱癌进展及与膀胱癌患者术后复发的相关性.方法 选取2014年6月-2019年12月于蚌埠医学院第一附属医院泌尿外科接受经尿道膀胱肿瘤等离子电切术(TURBT)的32例确诊为膀胱癌的患者.膀胱癌组织取自肿瘤所在部位区域,癌旁组织取自邻近正常区域组织;应用实时定量聚合酶链反应(qRT-P...

Correlation analysis of mir-15a and postoperative recurrence amongst patients with bladder carcinoma

Objective This study aimed to investigate the correlation between the miR-15 a expression level and progression of bladder cancer and postoperative recurrence in patients with bladder cancer.Methods Thirty-two patients diagnosed with bladder cancer who underwent transurethral plasma resection of bladder tumour transurethral resection of bladder tumour(TURBT)in the Department of Urology of the First Affiliated Hospital of Bengbu Medical College from June 2014 to December 2019 were enrolled in this study.Bladder cancer tissue was obtained from the tumour site,whereas paracancerous tissue was obtained from the adjacent normal area.Real-time quantitative polymerase chain reaction was used to detect the expression of miR-15 a in bladder cancer tissue samples or paracancerous tissue samples.The Kaplan-Meier method was used to describe the postoperative prognosis curve,and log-rank test was used for comparison amongst groups.Theχ~2-test test was used to compare the differences in the expression level and general clinical characteristics of miR-15 a in bladder cancer tissues and matched paracancerous tissues.Multivariate logistic regression analysis was used to describe the clinicopathological features such as muscular invasion,distant metastasis and tumour grade.Results The expression level of miR-15 a in bladder cancer tissues was significantly lower than that in adjacent tissues(1.126±0.254 vs 1.450±0.139),and the difference was statistically significant(P<0.001).In addition,the miR-15 a expression level was significantly correlated with muscular invasion(P=0.001),distant metastasis(P=0.005)and tumour grade(P=0.027).Log-rank test showed that patients with down-regulated miR-15 a expression had a higher overall recurrence rate,and the difference was statistically significant(P=0.023).Multivariate logistic regression analysis showed that positive distant metastasis,muscular invasion(T2-T4)and tumour grade G3 were independent risk factors for the down-regulation of miR-15 a expression.Conclusion The expression level of miR-15 a may be closely related to the occurrence and development of bladder cancer,and its expression level is significantly correlated with muscular invasion,distant metastasis and tumour grade.The down-regulated expression level of miR-15 a may be a biomarker for bladder tumour recurrence.