塞来昔布对成釉细胞瘤侵袭能力的影响及机制

目的 了解塞来昔布(CX)对成釉细胞瘤侵袭能力的影响,并对其作用机制进行初步探讨.方法 体外培养成釉细胞瘤(AB)原代细胞,采用MTT法检测细胞存活率,并根据MTT结果将AB原代细胞分为:CX高、中、低剂量组(140、70和35 μmol/L)、空白对照组和阳性对照组(阿霉素75 μmol/L);采用划痕试验法检测细胞...

Influence of celecoxib on invasion of ameloblastoma cells and its mechanism

Objective To explore the effects of celecoxib(CX)on the invasion of ameloblastoma(AB)cells and its mechanism of action.Methods The cell survival rate of primary-cultured AB cells in vitro was detected by MTT.According to the results of MTT,the AB cells were divided into three groups:CX high,medium and low dose groups(administered with CX 140,70 and 35μmol/L,respectively),blank control group(without processing)and positive control group(administered with adriamycin 75μmol/L).Cell migration and cell invasion were respectively measured by scratch test and modified Matrigel Boyden chamber assay.Western bloting was used to detect the protein expressions of matrix metalloproteinase(MMP)-2 and cyclooxygenase-2(COX-2).Results With the increase of CX dose,AB cell survival rate gradually decreased and were significantly lower in three CX groups than that in blank control group(P<0.05).Compared with those in blank control group,the cell migrations invasion,and the protein expressions of MMP-2 and COX-2 in CX groups and positive control group were significantly decreased(P<0.05).The above indexes in CX high dose group,middle dose group and positive control group were significantly lower than those in CX low dose group(P<0.05),while those in high dose group and positive control group were significantly lower than those in medium dose group(P<0.05).There were no significant differences in these indicators between CX high dose group and positive control group(P>0.05).Conclusion CX can effectively inhibit the invasion of ameloblastoma cells,which may be related to the protein expressions of MMP-2 and COX-2 inhibited by CX.