核糖体蛋白L29在舌鳞状细胞癌中的表达及意义

目的:探讨核糖体蛋白L29(ribosomal protein L29,RPL29)在人舌鳞状细胞癌(简称鳞癌)中的表达及意义.方法:应用免疫组织化学SP法检测52例舌鳞癌患者癌组织和癌旁组织中RPL29蛋白的表达情况.利用RNA干扰技术,将RPL29-siRNA转染到舌鳞癌CAL-27细胞中,RT-PCR法检测RPL...

Expression and significance of ribosomal protein L29 in tongue squamous cell carcinoma

PURPOSE:To investigate the expression and significance of ribosomal protein L29(RPL29)in human tongue squamous cell carcinoma(TSCC).METHODS:SP method of immunohistochemistry was carried out to detect the expression of RPL29 in cancer tissues and adjacent tissues of 52 TSCC patients.CAL-27 cells were transfected with RPL29-siRNA.RPL29 protein and mRNA levels were detected using Western blot and RT-PCR.Transwell invasion assay was performed to assess the invasion ability of CAL-27 cells.SPSS 21.0 software package was used for statistical analysis.RESULTS:The expression of RPL29 in TSCC was significantly higher than that in paracancerous tissues.RPL29 overexpression was found to be associated with cervical lymph node metastasis(P<0.05),but not with age,gender,smoking history,alcohol drinking history,pathological type and T stage(P>0.05).After transfecting RPL29-siRNA into CAL-27 cells,the ability of invasion was significantly decreased.CONCLUSIONS:The expression of RPL29 in TSCC was significantly increased and its expression level was significantly different from that in patients with or without lymph node metastasis.The invasive ability of CAL-27 cells was significantly inhibited after transfection with RPL29-siRNA.RPL29 may play an important role in the processes of invasion and metastasis of TSCC.