| HPLC 法测定甲硫酸新斯的明注射液的含量 |
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| 引用本文： | 孙莉. HPLC 法测定甲硫酸新斯的明注射液的含量[J]. 安徽医药, 2015, 0(1): 55-57. DOI: 10.3969/j.issn.1009-6469.2015.01.015 |
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| 作者姓名： | 孙莉 |
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| 作者单位： | 安徽省芜湖市食品药品检验所,安徽 芜湖,241001 |
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| 摘 要： | 目的：建立 HPLC 法测定甲硫酸新斯的明注射液的含量。方法用 Agilent ZORBAX Eclipse XDB C18（250 mm ×4．6 mm，5μm），以0．05 moL·L －1磷酸二氢钾溶液（用磷酸调节 pH 为3．0）—乙腈（90∶10）为流动相，流速为1．0 mL·min －1，检测波长为215 nm。结果甲硫酸新斯的明线性范围为在0．102～1．02 g·L －1（r ＝0．9999）；平均回收率为99．57％，RSD 为0．93％。结论该方法灵敏，快速，结果准确，可用于甲硫酸新斯的明注射液的质量控制。
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| 关 键 词： | HPLC 甲硫酸新斯的明注射液 含量 |
Determination of neostigmine methylsulfate injection by HPLC |
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| SUN Li. Determination of neostigmine methylsulfate injection by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2015, 0(1): 55-57. DOI: 10.3969/j.issn.1009-6469.2015.01.015 |
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| Authors: | SUN Li |
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| Affiliation: | SUN Li;Wuhu Institute for Food and Drug Control; |
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| Abstract: | Objective To establish a method for determination of neostigmine methylsulfate injection.Methods The column was Agi-lent ZORAX Eclipse XDB C18 column(250 mm ×4.6 mm,5 μm).The mobile phase was 0.05 moL·L -1 monopotassium phosphate solution(pH adjusted to 3.0 with phosphoric acid)-acetonitrile(90∶10).The flow rate was 1.0 mL·min -1 ,and the detection wave-length was 215 nm.Results The linear range of neostigmine methylsulfate was 0.102 ~1.02 g·L -1 (r =0.999 9).The average re-covery rate was 99.57% and RSD was 0.93%.Conclusions The method is sensitive,accurate and rapid,which can be used to deter-mine the content of neostigmine methylsulfate injection. |
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| Keywords: | HPLC neostigmine methylsulfate injection content |
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