多波长HPLC法同时测定复方黄白胶囊中3种成分的含量

目的:建立同时测定复方黄白胶囊中绿原酸、栀子苷和芍药苷3种成分含量的方法。方法:采用高效液相色谱法。色谱柱为ZORBAX Eclipse SB-C8(250 mm×4.6 mm,5μm),流动相为乙腈-2‰磷酸水溶液(梯度洗脱),流速为1.0 ml/min,柱温为30℃,绿原酸、栀子苷和芍药苷的检测波长分别为327、240和232 nm。结果:绿原酸、栀子苷和芍药苷的进样量分别在0.02⁰.19、0.090.19、0.09⁰.84和0.110.84和0.11¹.01μg范围内与各自峰面积积分值呈良好线性关系(r分别为0.999 7、0.999 8、0.999 9);精密度、稳定性、重复性试验的RSD<3%;平均加样回收率分别为99.6%、101.0%和100.2%,RSD分别为1.3%、0.7%和1.4%(n均为6)。结论:所建方法可用于复方黄白胶囊中3种成分的含量测定。

Simultaneous Determination of 3 Components in Compound Huangbai Capsules by Multi-wavelength HPLC Method

OBJECTIVE： To establish a method for simultaneous determination of chlorogenic acid, geniposide and paeoniflorin in Compound huangbai capsules. METHODS： HPLC method was adopted. The determination was performed on ZORBAX Eclipse SB-Cs （250 mm× 4.6 mm, 5 μm） column with acetonitrile-0.2%c phosphoric acid as mobile phase （gradient elution） at the flow rate of 1.0 ml/min. The column temperature was at 30 ℃. The detection wavelengths were set at 327, 240 and 232 nm for chlorogenic acid, geniposide and paeoniflorin, respectively. RESULTS： The linear ranges of chlorogenic acid, geniposide and paeoniflorin were 0.02-0.19 μg（r=0.999 7）, 0.09-0.84 μg（r=0.999 8） and 0.11-1.01 μg（r=0.999 9）, respectively. RSDs of precision, stability and reproducibility tests were all lower than 3%. The average recoveries of the 3 components were 99.6% （RSD=1.3% ,n=6）, 101.0% （RSD=0.7% ,n=6） and 100.2%（RSD=1.4% ,n=6）, respectively. CONCLUSIONS： The established method can be applied for the simultaneous determination of 3 components in Compound huangbai capsules.