米非司酮对人早孕期蜕膜细胞凋亡及其调控基因bcl-2/bax的影响

目的 :探讨米非司酮对早孕期蜕膜组织细胞bcl 2 /bax的影响及与妊娠终止的关系。方法 :用琼脂糖凝胶电泳、TUNEL法检测凋亡的发生及凋亡细胞的定位 ,用免疫组化法检测bcl 2 /bax蛋白的表达和相互关系。结果 :(1)正常早孕 4 0 + 天蜕膜组织细胞大量凋亡 ,bcl 2蛋白表达量较低 ,bax蛋白有较强表达 ;(2 )正常早孕 5 0 + 天 ,凋亡细胞明显减少 ,bcl 2的表达显著增强 ,bax蛋白表达减弱 ;(3)早孕 5 0 + 天应用米非司酮 ,蜕膜组织出现大量凋亡细胞及明显凋亡带 ,bcl 2蛋白表达明显降低 ,bax蛋白表达较前明显增强。结论 :早孕期米非司酮流产的机制可能与蜕膜组织细胞凋亡异常相关 ,bcl 2 /bax途径可能是其诱导早孕期蜕膜细胞凋亡的重要因素。

The effect of mifepristone on the apoptosis and the ratio of apoptotic gene bcl- 2/bax of decidua in human early gestational period.

Objective:To investigate the effect of mifepristone on the expression of bcl 2/bax in the decidua and relationship of failure of pregnancy in human early gestational period.Methods:The extent of DNA breakdown of apoptotic cells was assessed using an in site terminal transferase reaction to label free 3' ends of the DNA and electrophoresis on 2% agrose gel.The expressions of bcl 2 and bax in the decidua at various stages were examined by immunohistochemical staining with specific monoclonal antibodies.Results: (1)About 40 days of gestation,there was evident apoptosis in decidual tissue.The bcl 2 expression was low and bax was strongly expressed in the decidua.(2)About 50 days of gestation,there were a few apoptotic cells in the decidua.The bcl 2 expression increased greatly,but the expression of bax decreased.(3)More apoptotic cells were present in decidual tissue after administration of mifepristone in about 50 days of gestation and the bax protein expression was stimulated and bcl 2 protein was inhibited. Conclusions:(1)Apoptosis of decidua during gestation plays a significant role in blastocyst implantation and placental development.(2)The induction of apoptosis by mifepristone in decidual tissue may be an important mechanism in its contraceptive and contragestational effects.The channel of bcl 2/bax systems may be one of the mechanisms of mifepristone in decidua apoptosis.