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On the influence of reaction conditions in activity determination of alkaline phosphatase on the molar absorptivity of 4-nitrophenol |
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| Authors: | Klaus Jung Alfons Köhler |
| Affiliation: | 1. Department of Experimental Organ Transplantation at the Chair of Urology, University Hospital Charité, Humboldt University Berlin, Leninallee 49, DDR-1017 BerlinG.D.R.;2. Department of Experimental Organ Transplantation at the Chair of Urology, University Hospital Charité, Humboldt University Berlin City Hospital Strahlen, DresdenG.D.R. |
| Abstract: | In activity determination of alkaline phosphatase (AP), measuring temperature, type and concentration of buffer, and protein concentration in the test influence the molar absorptivity of 4-nitrophenol. Thus systematic errors of up to 3% may occur in activity determinations of AP if these influences are not taken into account. |
| Keywords: | Correspondence should be addressed to Chris Walker M.D. Director Clinical Chemistry Laboratory Section of Laboratory Medicine McMaster University Medical Center 1200 Main Street West Hamilton Ontario L8S 4J9 Canada. |
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