A simplified method for purification of the creatine kinase isoenzyme of human brain (BB) |
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| Authors: | FL Wang DW Cushman |
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| Institution: | Squibb Institute for Medical Research, Princeton, NJ 08540 U.S.A. |
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| Abstract: | A greatly simplified procedure has been developed for purification in high yields of creatine kinase isoenzyme BB from human brain. The procedure consists of fractional precipitation with ethanol, adsorption chromatography on hydroxylapatite, and fractional precipitation with ammonium sulfate. The essentially homogeneous enzyme obtained may be used as the antigen for radio immunoassay of blood isoenzyme MB of creatine kinase, which specifically increases following myocardial infarction. |
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| Keywords: | Correspondence should be addressed to D W Cushman Ph D E R Squibb & Sons Inc Squibb Institute for Medical Research P O Box 4000 Princeton NJ 08540 U S A |
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