抗人红细胞非凝集单克隆抗体的研制与应用

目的 研制出抗人红细胞非凝集单克隆抗体,进而构建双功能抗体试剂,用于临床实验室诊断.方法 用自愿者O型红细胞免疫BALB/C小鼠,采用淋巴细胞杂交瘤技术制备抗人红细胞非凝集单克隆抗体.结果 获得4株分泌抗人红细胞非凝集单克隆抗体的杂交瘤细胞株(7A1、7A2、1H7、7H3).4株单克隆抗体免疫球蛋白的轻链均为k型,重链均为IgG1类.检测200例正常人红细胞(A、AB、B、O型).Cooma's试验均发生凝集;与实验所用10种动物的红细胞无交叉反应.进一步对其在检测肾综合征出血热抗体的全血凝集试验方法方面的应用进行了初探.结论 研制的7A1抗人红细胞非凝集单克隆抗体可用于构建双功能试剂.

Study on Non-agglutination McAb to Human Red Blood Cell Antigen and i ts Application

Objective To obtain non-agglutination McAbs to human red blood cell antigens for constructing antibody reagents with two-function,which could be used in clinical diagnosis in lab.Methods BALB/c mice were immunized with human O group erythrocutes.Lymphocyte hybridoma technique was used to make McAb.Results Hybridoma cell lines(7A1,7A2,1H7,7H3)which could stably secrete McAbs against human red blood cell antigens were obtained.The isotype assay demonstrated that all the light chains of the McAbs were the K type and all the heavy chains were IgG1.The McAbs could specifically recognize the human red blood cell antigens on A,B,AB,O groups red blood cells from 200 donators respectively by Cooma's test and no cross-reaction was found with red blood cells from ten kinds of animals respectively.The application in full blood hemagglutination test for detecting hemorrhagic fever with renal syndrome(HFRS) was studied further.Conclusion The non-agglutination McAb(7Al) to human red blood cell antigen could be used to construct the antibody reagent with two -function.