热休克蛋白70对肝硬化大鼠肝脏缺血再灌注损伤保护作用的研究

目的 探讨热休克蛋白70（HSP70）对肝硬化大鼠肝脏缺血再灌注损伤的保护作用。方法Wistar雄性大鼠用四氯化碳皮下注射制成肝硬化模型。分两组:IP组（缺血预处理组）,用 Pringle’s法阻断肝门15min后,恢复血供,关腹;C组（对照组）,只予以开、关腹。48h后,再次阻断肝门30min,恢复血供。用Western blotting法检测IP后6、24、48h肝组织中HSP70的表达水平;测再灌注1h血清生化酶（ALT、AST、LDH）水平;计算术后一周生存率,并行肝脏病理组织学检查。结果 IP组在缺血预处理后24-48hHSP70表达显著增强,呈高水平;而C组中在各时点HSP70均无表达增强。再灌注1h,IP组的ALT、AST、LDH水平显著低于C组（P<0.01或P<0.05,n=7）。术后一周生存率IP组（93.10％,n=29）明显高于C组（73.33％,n=30）（P<0.05）。缺血再灌注后1h,IP组的肝细胞损伤明显轻于C组。结论 HSP70能够减轻肝硬化大鼠肝脏缺血再灌注损伤,提高术后生存率。

Experimental study on protective effects of heat shock protein 70 in ischemia- reperfusion injury of cirrhotic liver in rats

Objective To investigate the protective effects of HSP70 induced by ischemic pre - conditioning (IP) on ischemia - reperfusion injury (IRI) of cirrhotic liver in rats. Methods Male Wistar rats were used. The model of cirrhosis was produced by subcutaneous injection of carbon tetrachloride. Animals were divided into two groups: In IP group: Pringle' s manoeuvre was applied for 15 minutes. In C group of animals, the abdomen was only opened and closed. After 48 hours, Pringle s manoeuvre was maintained 30 minutes in all animals, then the inflow of the liver was resumed. HSP70 was measured with western blotting analysis. Results After 24, 48 hours of IP the HSP70 was kept at high levels in IP group. But, at the same time, it was hardly detected in C group. After 1 hour of reperfusion, the levels of ALT, AST, LDH in IP group were significantly lower than those found in C group ( P <0.01 or P <0.05); Furthermore, one week survival rate of IP group (93.10% ) was higher than that of C group (73.33% ) ( P < 0.05) and the damage of hepatocytes in IP group was less than that in C group. Conclusions In cirrhosis, HSP70 induced by IP had protective effects on IRI of liver. It could enhance the survival rate after operation.