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| 血管紧张素转换酶基因插入/缺失多态性改良法与经典法检测比较 | |
| 引用本文： | 梁舒,潘闽,管怀进,陈辉,崔之础. 血管紧张素转换酶基因插入/缺失多态性改良法与经典法检测比较[J]. 中国误诊学杂志, 2004, 4(9): 1388-1390 |
| 作者姓名： | 梁舒 潘闽 管怀进 陈辉 崔之础 |
| 作者单位： | 1. 江苏省南通医学院附属医院眼科,226001 2. 江苏省南通医学院附属医院心内科,226001 3. 江苏省南通医学院附属医院酶学实验室,226001 |
| 摘 要： | 目的 :研究血管紧张素转换酶 (ACE)插入 /缺失 (I/D)多态性的检测方法。方法 :用在 PCR反应系统中加入 5 %的二甲基亚砜 (DMSO)的改良法检测了 16 8例健康人群 ACE基因型 ,并与经典 Rigat方法进行比较。结果 :两种方法检测的 II基因型频率相同 ,但在 19例 DD基因型中 ,有 5例用 DMSO改良法检测后 ,却均为 ID基因型 ,误判率为2 6 .32 %。结论 :经典 Rigat法存在着较高率 ,改良法简便、可靠 ,适合临床实验室开展及大规模人群检测。 |
| 关 键 词： | 肽基二肽酶A/遗传学 基因缺失 多态现象(遗传学) 二甲亚砜 |
| 文章编号： | 1009-6647(2004)09-1388-03 |
| 修稿时间： | 2004-05-22 |
An Improved Method for Detecting Angiotensin Converting Enzyme(ACE) Gene Insert/Delete(I/D) Polymorphism Using Dimethyl Sulphoxide |
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| LIANG Shu,PAN Min,GUAN Huai-jin,et al.. An Improved Method for Detecting Angiotensin Converting Enzyme(ACE) Gene Insert/Delete(I/D) Polymorphism Using Dimethyl Sulphoxide[J]. Chinese Journal of Misdiagnostics, 2004, 4(9): 1388-1390 | |
| Authors: | LIANG Shu PAN Min GUAN Huai-jin et al. |
| Abstract: | Objective:To study the detecting method of ACE gene I/D polymorphism. Methods:The ACE genotypes of 168 cases were measured by classic Rigat method and an improved method by adding 5% dimethyl sulphoxide (DMSO) respectively,and the results were compared.Results:The two methods got the same II genotype frequency, but classic Rigat method had mistyping of ID genotype to DD genotype(26.32%).Conclusion:The improved method by adding 5% DMSO was a rapid and simple method for obtaining accurate and reliable result, and was suitable for clinic laboratory and mass population detected. |
| Keywords: | Peptidyl-dipeptidase A/genetics Gene deletion Polymorphism(genetics) Dimethyl sulphoxide |
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