蜈蚣提取液对肝癌Bel-7404细胞体外抗癌作用的研究

目的研究不同浓度蜈蚣提取液(ECP)对肝癌(HCC)Bel-7404细胞株的敏感性。方法通过MTT实验、细胞药物浓度—时间生长曲线研究不同浓度ECP对Bel-7404细胞株的敏感性;利用光学与电子显微镜观察细胞形态及其超微结构变化。结果①MTT法揭示各浓度ECP对人HCC细胞Bel-7404生长均有抑制作用。②细胞生长曲线图显示Bel-7404活细胞数目与ECP浓度及药物作用时间相关。ECP在5 g/L时,Bel-7404活细胞数先增多,48 h后细胞数开始减少;浓度在10 g/L与20 g/L时,用药后24 h活细胞数就显著减少。各组间活细胞数比较有显著性差异(P<0.01)。③光镜下见ECP作用48 h后,浓度低于5 g/L对Bel-7404细胞无明显的抑制作用,浓度在10 g/L以上时,Bel-7404活细胞数减少,细胞形态改变,细胞坏死。电镜下见治疗组细胞密度增高,染色质凝集固缩,染色质边集,核固缩、核碎裂,及凋亡小体的形成。结论①ECP能抑制人HCC细胞Bel-7404的增殖,且抑制作用呈剂量和时间依赖效应;②ECP低浓度有诱导肿瘤细胞凋亡作用,中、高浓度有直接杀肿瘤细胞作用。

Study of antitumous effect in vitro of extract of centipede on Bel-7404 cell of hepatocellular carcinoma

Objective It is to study the sensibility of different drug concentration extract of centipede(ECP) on Bel-7404 cell line of hepatocellular carcinoma(HCC).Methods The sensibility of different drug concentration ECP on Bel-7404 cell line was studied through MTT test and drug concentration-time growth curve.The cell morphous and the ultrastructural changes were observed through light microscope and electron microscope.Results ① MTT method revealed that different concentration ECP all had inhibitory action on the growth of HCC Bel-7404 cell line.② Cell growth curve chart showed that the viable counts of Bel-7404 had relation with ECP concentration and drug action time.When the concentration of ECP was 5 g/L,the viable counts of Bel-7404 increased firstly and then decreased gradually after 48 h.When the concentrations of ECP were 10 g/L and 20 g/L,the viable counts decreased significantly after medication for 24 h.There were significant differences on the viable counts among the groups(all P<0.01).③ Observed through light microscope after medication for 48 h,ECP had no obvious inhibitory action on Bel-7404 cell when the concentration was lower than 5 g/L.When the concentration was higher than 10 g/L,the viable counts of Bel-7404 decreased,the cell morphous transformed and neorobiosis.Through electron microscope in treatment group,the cell density heightened,chromatic agglutination and pycnosis,chromatin margination,karyopyknosis,nuclear fragmentation and apoptotic body formation were observed.Conclusion ① ECP can inhibit the proliferation of HCC Bel-7404.The inhibitory action is time-concentration dependent.② Light concentration of ECP has the action of inducing tumor cell apoptosis.Middle and high concentrations of ECP has the action of direct killing tumor cell.