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| 黑素转铁蛋白在家兔网织红细胞非转铁蛋白结合铁摄取中的作用 | |
| 引用本文： | 陈文芳,钱忠明.黑素转铁蛋白在家兔网织红细胞非转铁蛋白结合铁摄取中的作用[J].中国病理生理杂志,2000,16(12):1317-1319. |
| 作者姓名： | 陈文芳 钱忠明 |
| 作者单位： | 1. 青岛大学医学院生理教研室, 山东 青岛 266021; 2. 香港理工大学应用生物及化学技术系 |
| 摘 要： | 目的:研究黑素转铁蛋白(P97)在家兔网织红细胞非转铁蛋白结合铁摄取中的作用。方法:放射性同位素法(59Fe])。结果:(1)单纯用磷脂酰肌醇磷脂酶C(PI-PLC)作用网织红细胞,其铁摄取无明显变化(P>0.05);(2)经蛋白酶procnase预处理后,再用PI-PLC作用网织红细胞,则胞浆内铁及整合到血红素中的铁均较单纯用蛋白酶处理的网织红细胞明显降低。结论:在家兔网织红细胞膜上可能存在有P97,且P97在其摄取非转铁蛋白结合铁的过程中可能发挥作用。 |
| 关 键 词： | 转铁蛋白 铁 网状细胞 |
| 文章编号： | 1000-4718(2000)12-1317-03 |
| 收稿时间： | 1999-08-05 |
| 修稿时间： | 1999年8月5日 |
Role of P97 in non- transferrin bound iron uptake by rabbit reticulocytes |
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| CHEN Wen-fang,Qian Zhong-ming.Role of P97 in non- transferrin bound iron uptake by rabbit reticulocytes[J].Chinese Journal of Pathophysiology,2000,16(12):1317-1319. | |
| Authors: | CHEN Wen-fang Qian Zhong-ming |
| Institution: | 1. Department of Physiology, The Medical College of Qingdao University, Qingdao 266021, China; 2. Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, China |
| Abstract: | AIM: To investigate the possible role of P97 in non-transferrin bound iron uptake by rabbit reticulocytes. METHODS: The iron uptake were measured by the method of radioisotope ( 59Fe). RESULTS: (1) Only PI-PLC treatment had no apparent effect on iron uptake by reticulocytes (P>0.05); (2) Reticulocytes, pretreated by pronase and then by PI-PLC, give a significant decrease in iron uptake in cytosol and in heme (P<0.01). CONCLUSION: The results support the possibility that P97 might be able to be expressed on the membrane of reticulocytes and plays a role in non-transferrin bound iron uptake by this type of cells in the rabbit. |
| Keywords: | Transferrin Iron Reticulocytes |
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