促红细胞生成素及其受体在卵巢癌细胞的表达及相关作用的实验研究

 目的　检测卵巢癌细胞中的促红细胞生成素（EPO）及其受体（EPOR）表达，以及重组人EPO（rhEPO）对卵巢癌细胞增生的影响，探讨rhEPO在癌性贫血治疗中的意义。方法　RT-PCR方法检测人类卵巢癌细胞株 HO-8910 EPOR mRNA的表达。用流式细胞仪检测rhEPO对卵巢癌细胞凋亡及增生影响。结果　卵巢癌细胞表面有EPOR的表达，rhEPO干预后卵巢癌细胞增生受到抑制（P＜0.01）。结论　EPOR在卵巢癌细胞株HO-8910有表达，rhEPO可抑制卵巢癌细胞的增生。rhEPO可用于卵巢癌伴发的贫血的治疗。

Expression and related function of erythropoietin and its receptor in ovarian epithelial tumor

Objective To detect the expression of EPOR in ovarian epithelial tumor. Study the biologic effect of rHuEPO to ovarian epithelial tumor cell H08910. Methods RT-PCR analyses were used to detect the expression of EPOR in ovarian epithelial tumor cell, FCM assay was used to detect rHuEPOs effect to ovarian epithelial tumor cell proliferation and inhibition ratio. Results There is EPOR expressions in ovarian epithelial tumor cell. rHuEPO could reduce ovarian epithelial tumor cell expressions (P <0.01). Conclusion The lower expression of EPOR was observed in ovarian epithelial tumor cell. Use rHuEPO couldn't promote the cancer's progression and bring negative effect in clinical practice. Further clinical research is required to prove it.