| 乙型肝炎病毒基因生物信息学分析及特异片段的制备 |
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| 引用本文： | 徐晓雪,房凯,钟连声,潘忠诚,李超,胥威,赵雨杰.乙型肝炎病毒基因生物信息学分析及特异片段的制备[J].中国医科大学学报,2008,37(5). |
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| 作者姓名： | 徐晓雪 房凯 钟连声 潘忠诚 李超 胥威 赵雨杰 |
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| 作者单位： | 中国医科大学,基础医学院生物芯片中心,沈阳,11000 |
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| 基金项目： | 辽宁省教育厅高校科研基金 |
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| 摘 要： | 目的制备HBV基因分型芯片模板。方法通过不同基因型HBV全基因组的序列比对,选取6个碱基位点作为分型位点,应用重叠区扩增基因拼接法(SOEing法)对C型HBV片段进行点突变。结果在所选位点上成功引入点突变。结论SOEing法是一种简便引入点突变的方法,可以应用此法制备HBV基因分型检测基因所需的模板DNA。
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| 关 键 词： | 重叠区扩增基因拼接法 乙型肝炎病毒 基因分型 |
Bioinformatic Analysis of HBV Gene and Preparation of Specific Fragment |
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| XU Xiao-xue,FANG Kai,ZHONG Lian-sheng,PAN Zhong-cheng,LI Chao,XU Wei,ZHAO Yu-jie.Bioinformatic Analysis of HBV Gene and Preparation of Specific Fragment[J].Journal of China Medical University,2008,37(5). |
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| Authors: | XU Xiao-xue FANG Kai ZHONG Lian-sheng PAN Zhong-cheng LI Chao XU Wei ZHAO Yu-jie |
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| Abstract: | Objective To prepare HBV DNA fragments for HBV genotypes used as templates. Methods Six bases were chosen for genotyping by comparing different HBV genome sequences. The fragments of HBV genotype C were mutated by using gene splicing by overlap extension (gene SOEing). Results The chosen sites were successfully mutated. Conclusion Gene SOEing is a convenient way to introduce mutation,and this method can be used to prepare HBV specific sequences that can be used as templates. |
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| Keywords: | gene splicing by overlap extension HBV genotyping |
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