前列地尔对培养乳鼠心肌细胞缺氧复氧损伤的保护作用

目的 :观察前列地尔对培养乳鼠心肌细胞缺氧复氧损伤的保护作用。方法 :采用纯化培养的心肌细胞建立缺氧复氧损伤模型 ,观察不同浓度前列地尔 (5 ,15 ,4 5 μg·L- 1)剂量组细胞的形态学变化 ,黄嘌呤氧化酶法测定细胞内超氧化物歧化酶(SOD)活力 ,硫代巴比妥酸显色法测定细胞内丙二醛 (MDA)含量 ,并测定缺氧复氧后细胞培养液中乳酸脱氢酶 (LDH)活性 ,透射电镜观察细胞超微结构改变。结果 :与正常组比较 ,模型组细胞SOD下降 ,MDA和LDH升高 (均P <0 .0 1)。前列地尔各剂量组与模型组比较 ,心肌细胞SOD(除小剂量组 )活性提高 ,MDA和LDH含量降低 (P <0 .0 1)。且心肌细胞形态及超微结构改善。结论 :前列地尔具有明显的抗缺氧复氧损伤、保护心肌细胞的作用。

Protective effects of alprostadil on hypoxia-reoxygenation injury in cultured neonatal rat myocardial cells

AIM: To study the protective effects of alprostadil on hypoxia-reoxygenation(H-R) injury in cultured neonatal rat myocardial cells. METHODS: The H-R injury was induced in cultured neonatal rat myocardial cells. And the myocardial cells were cultured with different concentrations of alprostadil (5,15,45 μg·L -1,little,middle,large concentration group). The morphological changes of each group myocardial cells were observed. The activities of plasma superoxide dismutase(SOD) were measured by the method of xanthine oxidase and the contents of serum malonicaldehyde(MDA) by the method of thiobarbituric acid.The activities of lactate dehydrogenase(LDH) in cell-cultured solution after H-R injury were measured. The ultrastructural features of cardiomyocytes were observed by electron microscope. RESULTS:Compared with normal group,SOD activity decreased and MDA,LDH increased in model group ( P < 0.01 ).Compared with model group,SOD activity increased (except little concentration group),MDA and LDH decreased ( P <0.01) in each alprostadil group. And the morphological and ultrastructural changes of cardiomyocytes were improved. CONCLUSION: Alprostadil has protective effects on cultured neonatal rat myocardial cells injured by H-R.