| 孕期PFOS暴露胎鼠肺相关基因表达 |
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| 引用本文： | 张春雷,柏合,张哲,韩鹏飞,赵冰海,初彦辉. 孕期PFOS暴露胎鼠肺相关基因表达[J]. 牡丹江医学院学报, 2013, 0(4): 5-7 |
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| 作者姓名： | 张春雷 柏合 张哲 韩鹏飞 赵冰海 初彦辉 |
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| 作者单位： | [1]牡丹江医学院医药研究中心,黑龙江牡丹江157011 [2]牡丹江医学院附属红旗医院检验科,黑龙江牡丹江157011 |
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| 基金项目： | 黑龙江省卫生厅科研课题项目（2010-235,2010-238）;牡丹江医学院科学技术研究项目（2011-16） |
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| 摘 要： | 目的：研究孕期PFOS暴露胎鼠肺损伤中相关基因的表达。方法：通过3种剂量的PFOS对孕期SD大鼠处理7天,采取胎鼠全肺分析。结果：通过形态学比较,发现PFOS暴露导致胎鼠体长、体重均显著降低（p〈0.05）,高剂量暴露会导致胎鼠死亡。通Real-time PCR检测,发现胎鼠肺Hsd11β1显著升高（p〈0.01）,Hsd11β2显著降低（p〈0.05）,H6pdh显著升高（p〈0.01）,Vegfa显著升高（p〈0.01）。结论：实验结果表明孕期PFOS暴露会导致胎鼠发育抑制、肺脏发育受损,其相关基因表达量出现显著变化。
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| 关 键 词： | 全氟辛烷磺酸 胎鼠 血管内皮生长因子 发育抑制 |
THE EXPRESSION OF RELATED GENE IN LUNG OF ENBRYONIC RAT EXPOSED TO PFOS DURING PREGNANCY |
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| Affiliation: | ZHANG Chunlei et al ( Mudanjiang Medical University Medical Research Center, Mudanjiang 157011 ,China) |
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| Abstract: | Objective：Investigated the expression of related gene in lung impairments of embryonic rat induced by prenatal PFOS exposure, nethods：SD rats were exposed to PFOS at 3 dosages for 7days, the whole pups lung was collected to analysis. Results：Mor- phologically, the length and the weights of embryos significantly decreased （ p 〈 O. 05 ） after prenatal SD rats exposed to PFOS in a dose -dependent manner, higher dose PFOS caused death of embryonic rat. The expression of related gene in embryonic rat were detected by Real-time PCR, compared to the control, Hsdlll31 was significantly increased （p 〈 0. O1 ）, Hsdll[~2 was significantly de- creased （ p 〈 0.05）, H6pdh was significantly increased （ p 〈 0. 01 ）, Vegfa was significantly increased （ p 〈 0. 01 ）. Conclusion： The results showed prenatal PFOS exposure caused embryonic rat growth inhibition and lung impairments, the expression of related gene in embryonic rat lung was significantly changed. |
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| Keywords: | Perfluorooctanesulfonate Pups VEGF Growth inhibition |
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